Anti-Xyloglucan [CCRC-M39] Antibody (supernatant)
![Anti-Xyloglucan [CCRC-M39] Antibody (supernatant)](https://www.kerafast.com/MediaStorage/Product/Images/Large/1288_2001202001264112870.jpg "Anti-Xyloglucan [CCRC-M39] Antibody (supernatant)")
This mouse IgA monoclonal antibody was generated against xyloglucan and recognizes sycamore, lupin and gum tragacanth fucosylated xyloglucans and rhamnogalacturonan I (RG1).
Highlights:
- Reacts with sycamore, lupin and gum tragacanth fucosylated xyloglucans and RG1
- CCRC-M39 binds strongly to fucosylated xyloglucans and to rhamnogalacturonan I from sycamore maple
- Suitable for ELISA applications
Xyloglucan is a hemiceullose or polysaccharide that is found in the primary cell wall of all vascular plants. Xyloglucan binds to the surface of cellulose microfibrils and may link them together. Rhamnogalacturonans (RGs) are a family of related cell wall pectic polysaccharides that contain a repeating backbone of a-D-GalpA-(1,2)-a-L-Rhap-(1).
From the laboratory of Michael G. Hahn, PhD, University of Georgia.
This mouse IgA monoclonal antibody was generated against xyloglucan and recognizes sycamore, lupin and gum tragacanth fucosylated xyloglucans and rhamnogalacturonan I (RG1).
Highlights:
- Reacts with sycamore, lupin and gum tragacanth fucosylated xyloglucans and RG1
- CCRC-M39 binds strongly to fucosylated xyloglucans and to rhamnogalacturonan I from sycamore maple
- Suitable for ELISA applications
Xyloglucan is a hemiceullose or polysaccharide that is found in the primary cell wall of all vascular plants. Xyloglucan binds to the surface of cellulose microfibrils and may link them together. Rhamnogalacturonans (RGs) are a family of related cell wall pectic polysaccharides that contain a repeating backbone of a-D-GalpA-(1,2)-a-L-Rhap-(1).
From the laboratory of Michael G. Hahn, PhD, University of Georgia.
| Product Type: | Antibody |
| Antigen: | Zea maize4-O-methylglucuronoxylan/MeBSA |
| Isotype: | IgA |
| Clonality: | Monoclonal |
| Clone Name: | 4E1.E7.A2.A4 |
| Reactivity: | Sycamore, lupin, gum tragacanth |
| Immunogen: | Xyloglucan |
| Species Immunized: | Mouse |
| Buffer: | Cell culture supernatant |
| Tested Applications: | ELISA (undiluted to 1:10) |
| Storage: | <1 month at 4C, >1 month at -80C |
| Shipped: | Cold Packs |
These monoclonal antibodies were developed under the sponsorship of the US National Science Foundation, through award number DBI-0421683. Their use in biomass characterization, study of biomass deconstruction and quantitation was developed under the sponsorship of the US Department of Energy through awards DE-PS02-06ER64304 and DE-AC05-00OR22725 (BioEenergy Science Center).
- Robin E. Young, Heather E. McFarlane, Michael G. Hahn, Tamara L. Western, George W. Haughn and A. Lacey Samuels. 2008. Analysis of the Golgi Apparatus in Arabidopsis Seed Coat Cells during Polarized Secretion of Pectin-Rich Mucilage. The Plant Cell June 2008 vol. 20 no. 6 1623-1638 .
- DeMartini, JD, Pattathil, S, Avci, U, Szekalski, K, Mazumder, K, Hahn, M.G., Wyman, CE: Application of monoclonal antibodies to investigate plant cell wall deconstruction for biofuels production. Energy Environ. Sci., 2011, 4, 4332-4339.
- Pattathil S, Avci U, Miller JS, Hahn MG. 2012. Immunological approaches to plant cell wall and biomass characterization: Glycome profiling. In: Himmel M (ed) Biomass Conversion: Methods and Protocols. Springer Science + Business Media, LLC, New York, NY, pp 61-72.
- A.P. de Souza, D.C.C. Leite, S. Pattahil, M.G. Hahn, M.S. Buckeridge. 2013. Composition and structure of sugarcane cell wall polysaccharides: Implications for second generation bioethanol production. Bioenergy Research 6: 564-579.
- J. Puhlmann, E. Bucheli, M. J. Swain, N. Dunning, P. Albersheim, A. G. Darvill, and M. G. Hahn. (1994) Generation of monoclonal antibodies against plant cell wall polysaccharides. I. Characterization of a monoclonal antibody to a terminal alpha-(1,2)-linked fucosyl-containing epitope. Plant Physiol. 104:699-710.
- G. Freshour, R. P. Clay, M. S. Fuller, P. Albersheim, A. G. Darvill, and M. G. Hahn. (1996) Developmental and tissue-specific structural alterations of the cell-wall polysaccharides of Arabidopsis thaliana roots. Plant Physiol. 110:1413-1429.
- G. Freshour, C. P. Bonin, W.-D. Reiter, P. Albersheim, A. G. Darvill, and M. G. Hahn. (2003) Distribution of fucose-containing xyloglucans in cell walls of the mur1 mutant of Arabidopsis thaliana. Plant Physiol. 131:1602-1612.
- Pattathil S, Avci U, Baldwin D, Swennes AG, McGill JA, Popper Z, Bootten T, Albert A, Davis RH, Chennareddy C, Dong R, O'Shea B, Rossi R, Leoff C, Freshour G, Narra R, O'Neil M, York WS, Hahn MG. (2010) A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies. Plant Physiol. 153:514-525.
- Pattathil S, Avci U, Miller JS, Hahn MG. 2012. Immunological approaches to plant cell wall and biomass characterization: Glycome profiling. In: Himmel M (ed) Biomass Conversion: Methods and Protocols. Springer Science + Business Media, LLC, New York, NY, pp 61-72.
- Pattathil S, Avci U, Baldwin D, et al. 2010. A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiology 153, 514-525.
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