Anti-Linseed mucilage [CCRC-M141] Antibody (supernatant)
![Anti-Linseed mucilage [CCRC-M141] Antibody (supernatant)](https://www.kerafast.com/MediaStorage/Product/Images/Large/1303_2001202001264213020.jpg "Anti-Linseed mucilage [CCRC-M141] Antibody (supernatant)")
This mouse IgG2b monoclonal antibody was generated against seed mucilage/MeBSA complex and is specific for linseed mucilage.
Highlights:
- Reacts with linseed mucilage
- Suitable for ELISA, Immunolabeling and Immmunofluorescence applications
Mucilage is found on nearly all plants and some micoorganisms and plays a role in the storage of water and food, thickening membranes and seed germination. The substance is a thick, viscous, gley substance and is a polar glycoprotein and exopolysaccharide.
From the laboratory of Michael G. Hahn, PhD, University of Georgia.
This mouse IgG2b monoclonal antibody was generated against seed mucilage/MeBSA complex and is specific for linseed mucilage.
Highlights:
- Reacts with linseed mucilage
- Suitable for ELISA, Immunolabeling and Immmunofluorescence applications
Mucilage is found on nearly all plants and some micoorganisms and plays a role in the storage of water and food, thickening membranes and seed germination. The substance is a thick, viscous, gley substance and is a polar glycoprotein and exopolysaccharide.
From the laboratory of Michael G. Hahn, PhD, University of Georgia.
| Product Type: | Antibody |
| Accession ID: | CCRC M141 |
| Antigen: | Linseed mucilage |
| Isotype: | IgG2b |
| Clonality: | Monoclonal |
| Clone Name: | CCRC M141 |
| Reactivity: | Linseed mucilage |
| Immunogen: | Seed Mucilage/MeBSA |
| Species Immunized: | Mouse |
| Buffer: | Cell culture supernatant |
| Tested Applications: | ELISA, Immunolableling, IF |
| Storage: | <1 month at 4C, >1 month at -80C |
| Shipped: | Cold Packs |
CCRC-M141 Cross Reactivity Map
CCRC-M141 binds only to Linseed mucilage among the 54 polysaccharides tested. (very high specificity).
These monoclonal antibodies were developed under the sponsorship of the US National Science Foundation, through award number DBI-0421683. Their use in biomass characterization, study of biomass deconstruction and quantitation was developed under the sponsorship of the US Department of Energy through awards DE-PS02-06ER64304 and DE-AC05-00OR22725 (BioEenergy Science Center).
- Robin E. Young, Heather E. McFarlane, Michael G. Hahn, Tamara L. Western, George W. Haughn and A. Lacey Samuels. 2008. Analysis of the Golgi Apparatus in Arabidopsis Seed Coat Cells during Polarized Secretion of Pectin-Rich Mucilage. The Plant Cell June 2008 vol. 20 no. 6 1623-1638 .
- DeMartini, JD, Pattathil, S, Avci, U, Szekalski, K, Mazumder, K, Hahn, M.G., Wyman, CE: Application of monoclonal antibodies to investigate plant cell wall deconstruction for biofuels production. Energy Environ. Sci., 2011, 4, 4332-4339.
- Pattathil S, Avci U, Miller JS, Hahn MG. 2012. Immunological approaches to plant cell wall and biomass characterization: Glycome profiling. In: Himmel M (ed) Biomass Conversion: Methods and Protocols. Springer Science + Business Media, LLC, New York, NY, pp 61-72.
- A.P. de Souza, D.C.C. Leite, S. Pattahil, M.G. Hahn, M.S. Buckeridge. 2013. Composition and structure of sugarcane cell wall polysaccharides: Implications for second generation bioethanol production. Bioenergy Research 6: 564-579.
- J. Puhlmann, E. Bucheli, M. J. Swain, N. Dunning, P. Albersheim, A. G. Darvill, and M. G. Hahn. (1994) Generation of monoclonal antibodies against plant cell wall polysaccharides. I. Characterization of a monoclonal antibody to a terminal alpha-(1,2)-linked fucosyl-containing epitope. Plant Physiol. 104:699-710.
- G. Freshour, R. P. Clay, M. S. Fuller, P. Albersheim, A. G. Darvill, and M. G. Hahn. (1996) Developmental and tissue-specific structural alterations of the cell-wall polysaccharides of Arabidopsis thaliana roots. Plant Physiol. 110:1413-1429.
- G. Freshour, C. P. Bonin, W.-D. Reiter, P. Albersheim, A. G. Darvill, and M. G. Hahn. (2003) Distribution of fucose-containing xyloglucans in cell walls of the mur1 mutant of Arabidopsis thaliana. Plant Physiol. 131:1602-1612.
- Pattathil S, Avci U, Baldwin D, Swennes AG, McGill JA, Popper Z, Bootten T, Albert A, Davis RH, Chennareddy C, Dong R, O'Shea B, Rossi R, Leoff C, Freshour G, Narra R, O'Neil M, York WS, Hahn MG. (2010) A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies. Plant Physiol. 153:514-525.
- Pattathil S, Avci U, Miller JS, Hahn MG. 2012. Immunological approaches to plant cell wall and biomass characterization: Glycome profiling. In: Himmel M (ed) Biomass Conversion: Methods and Protocols. Springer Science + Business Media, LLC, New York, NY, pp 61-72.
- Pattathil S, Avci U, Baldwin D, et al. 2010. A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiology 153, 514-525.
- Pattathil S, Hahn MG, Dale BE, Chundawat SP. Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEXTM-pre-treated biomass. J Exp Bot. 2015 Jul;66(14):4279-94.
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