Mouse embryonic fibroblast cell line expressing wild-type human B-cell lymphoma-extra-large (Bcl-xL).
Also available: Mouse Embryonic Fibroblast Bcl-xL Knock Out Cell Line (MEF Bcl-xL-KO)
B-cell lymphoma 2 (Bcl-2) proteins are major regulators of cellular responses to intrinsic and extrinsic apoptotic stimuli. Among them, overexpression of the antiapoptotic protein Bcl-xL modulates intracellular Ca2+ homeostasis and organelle-specific apoptotic signaling pathways. Bcl-xL is a transmembrane molecule in the mitochondria and acts as a pro-survival protein by preventing the release of mitochondrial contents such as cytochrome c, which would lead to caspase inactivation. Relative amounts of pro- and anti-survival Bcl-2 family of proteins define whether the cell will undergo cell death. If more Bcl-xL is present, then pores are non-permeable to pro-apoptotic molecules and the cell survives. However, if Bcl-2 members Bax and Bak become activated, and Bcl-xL is sequestered away by gatekeeper BH3-only factors (e.g., Bim), causing a pore to form, cytochrome c is released leading to initiation of caspase cascade leading to apoptotic events.
From the laboratory of Chi Li, PhD, University of Louisville.
Product Type: | Cell Line |
Name: | MEF wild-type |
Cell Type: | Fibroblast |
Accession ID: | Q07817,CVCL_HC61 |
Source: | Embryos |
Organism: | Murine |
Morphology: | Cells are bipolar or multipolar, have elongated shapes, and grow attached to a substrate |
Biosafety Level: | BSL2 |
Growth Conditions: | DMEM + 10% fetal bovine serum |
Subculturing: | 1/10 split |
Cryopreservation: | fetal bovine serum + 10% DMSO |
Storage: | Liquid nitrogen |
Shipped: | Dry ice |
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