Anti-Xyloglucan [CCRC-M49] Antibody (supernatant)
![Anti-Xyloglucan [CCRC-M49] Antibody (supernatant)](https://www.kerafast.com/MediaStorage/Product/Images/Large/1355_2001202001264413540.jpg "Anti-Xyloglucan [CCRC-M49] Antibody (supernatant)")
This mouse IgG1 monoclonal antibody was generated against xyloglucan/BSA covalent conjugate and recognizes tamarind, tomato and linseed xyloglucan.
Highlights:
- Reacts with tamarind, tomato and linseed xyloglucanBinds to an epitope on non-fucosylated xyloglucans
- Does not bind to oligosaccharides isolated from xyloglucan, suggesting that it binds to a conformational feature of the intact polysaccharide or to an epitope that encompasses more than one xyloglucan subunit
- Suitable for ELISA applications
Xyloglucan is a hemiceullose or polysaccharide that is found in the primary cell wall of all vascular plants. Xyloglucan binds to the surface of cellulose microfibrils and may link them together.
From the laboratory of Michael G. Hahn, PhD, University of Georgia.
This mouse IgG1 monoclonal antibody was generated against xyloglucan/BSA covalent conjugate and recognizes tamarind, tomato and linseed xyloglucan.
Highlights:
- Reacts with tamarind, tomato and linseed xyloglucanBinds to an epitope on non-fucosylated xyloglucans
- Does not bind to oligosaccharides isolated from xyloglucan, suggesting that it binds to a conformational feature of the intact polysaccharide or to an epitope that encompasses more than one xyloglucan subunit
- Suitable for ELISA applications
Xyloglucan is a hemiceullose or polysaccharide that is found in the primary cell wall of all vascular plants. Xyloglucan binds to the surface of cellulose microfibrils and may link them together.
From the laboratory of Michael G. Hahn, PhD, University of Georgia.
Specifications
| Product Type: | Antibody |
| Accession ID: | CCRC M49 |
| Antigen: | Xyloglucan of Tamarindus indicus |
| Isotype: | IgG1 |
| Clonality: | Monoclonal |
| Clone Name: | 12H12.E3.F3.Ce2H12.E3.F3.C3 |
| Reactivity: | Tamarind, tomato, linseed |
| Immunogen: | Xyloglucan/BSA covalent conjugate |
| Species Immunized: | Mouse |
| Buffer: | Cell culture supernatant |
| Tested Applications: | ELISA |
| Storage: | <1 month at 4C, >1 month at -80C |
| Shipped: | Cold Packs |
Documentation
Notes:
These monoclonal antibodies were developed under the sponsorship of the US National Science Foundation, through award number DBI-0421683. Their use in biomass characterization, study of biomass deconstruction and quantitation was developed under the sponsorship of the US Department of Energy through awards DE-PS02-06ER64304 and DE-AC05-00OR22725 (BioEenergy Science Center).
Provider
From the laboratory of Michael G. Hahn, PhD, University of Georgia.
References
- Robin E. Young, Heather E. McFarlane, Michael G. Hahn, Tamara L. Western, George W. Haughn and A. Lacey Samuels. 2008. Analysis of the Golgi Apparatus in Arabidopsis Seed Coat Cells during Polarized Secretion of Pectin-Rich Mucilage. The Plant Cell June 2008 vol. 20 no. 6 1623-1638 .
- DeMartini, JD, Pattathil, S, Avci, U, Szekalski, K, Mazumder, K, Hahn, M.G., Wyman, CE: Application of monoclonal antibodies to investigate plant cell wall deconstruction for biofuels production. Energy Environ. Sci., 2011, 4, 4332-4339.
- Pattathil S, Avci U, Miller JS, Hahn MG. 2012. Immunological approaches to plant cell wall and biomass characterization: Glycome profiling. In: Himmel M (ed) Biomass Conversion: Methods and Protocols. Springer Science + Business Media, LLC, New York, NY, pp 61-72.
- A.P. de Souza, D.C.C. Leite, S. Pattahil, M.G. Hahn, M.S. Buckeridge. 2013. Composition and structure of sugarcane cell wall polysaccharides: Implications for second generation bioethanol production. Bioenergy Research 6: 564-579.
- J. Puhlmann, E. Bucheli, M. J. Swain, N. Dunning, P. Albersheim, A. G. Darvill, and M. G. Hahn. (1994) Generation of monoclonal antibodies against plant cell wall polysaccharides. I. Characterization of a monoclonal antibody to a terminal alpha-(1,2)-linked fucosyl-containing epitope. Plant Physiol. 104:699-710.
- G. Freshour, R. P. Clay, M. S. Fuller, P. Albersheim, A. G. Darvill, and M. G. Hahn. (1996) Developmental and tissue-specific structural alterations of the cell-wall polysaccharides of Arabidopsis thaliana roots. Plant Physiol. 110:1413-1429.
- G. Freshour, C. P. Bonin, W.-D. Reiter, P. Albersheim, A. G. Darvill, and M. G. Hahn. (2003) Distribution of fucose-containing xyloglucans in cell walls of the mur1 mutant of Arabidopsis thaliana. Plant Physiol. 131:1602-1612.
- Pattathil S, Avci U, Baldwin D, Swennes AG, McGill JA, Popper Z, Bootten T, Albert A, Davis RH, Chennareddy C, Dong R, O'Shea B, Rossi R, Leoff C, Freshour G, Narra R, O'Neil M, York WS, Hahn MG. (2010) A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies. Plant Physiol. 153:514-525.
- Pattathil S, Avci U, Miller JS, Hahn MG. 2012. Immunological approaches to plant cell wall and biomass characterization: Glycome profiling. In: Himmel M (ed) Biomass Conversion: Methods and Protocols. Springer Science + Business Media, LLC, New York, NY, pp 61-72.
- Pattathil S, Avci U, Baldwin D, et al. 2010. A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiology 153, 514-525.
- Pattathil S, Avci U, Baldwin D, et al. 2010. A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiology 153, 514-525.
- Pattathil S, Hahn MG, Dale BE, Chundawat SP. Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEXTM-pre-treated biomass. J Exp Bot. 2015 Jul;66(14):4279-94.
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