Anti-Porcine Rotavirus VP6, Serogroup C [11H3] Antibody
![Anti-Porcine Rotavirus VP6, Serogroup C [11H3] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1420_2001202001264714190.jpg "Anti-Porcine Rotavirus VP6, Serogroup C [11H3] Antibody")
This mouse IgG monoclonal antibody was generated against purified recombinant porcine rotavirus (PoRV) VP6 subclass C and is specific for porcine PoRV VP6 subclass C.
Highlights:
- Recognizes porcine PoRV VP6 subclass C
- Suitable for Immunofluorescence, Immunohistochemistry, Western Blot and ELISA applications
Porcinerotavirus (PoRV) is a non-enveloped icosahedral virus belonging to the genus Rotavirus and family Rotaviridae. Rotavirus genome has 11 segments which encode 6 non-structure proteins(NSP 1, 2, 3, 4, 5, 6) and 6 structure proteins (VP 1, 2, 3, 4 ,6, 7). Virion has three layers. The outer layer is comprised of VP4 and VP7 which are necessary for virus entry and involved in virus neutralization. The middle layer is VP6 protein,the most abundant and conserve structure protein. Base on the antigenicity and sequence of VP6, rotaviruses are divided into 8 serogroups (A-H). Serogroup A, B, C, E and H have been implicated in swine enteric disease.
From the laboratory of
Kyoungjin J. Yoon, DVM, PhD, Iowa State University.
This mouse IgG monoclonal antibody was generated against purified recombinant porcine rotavirus (PoRV) VP6 subclass C and is specific for porcine PoRV VP6 subclass C.
Highlights:
- Recognizes porcine PoRV VP6 subclass C
- Suitable for Immunofluorescence, Immunohistochemistry, Western Blot and ELISA applications
Porcinerotavirus (PoRV) is a non-enveloped icosahedral virus belonging to the genus Rotavirus and family Rotaviridae. Rotavirus genome has 11 segments which encode 6 non-structure proteins(NSP 1, 2, 3, 4, 5, 6) and 6 structure proteins (VP 1, 2, 3, 4 ,6, 7). Virion has three layers. The outer layer is comprised of VP4 and VP7 which are necessary for virus entry and involved in virus neutralization. The middle layer is VP6 protein,the most abundant and conserve structure protein. Base on the antigenicity and sequence of VP6, rotaviruses are divided into 8 serogroups (A-H). Serogroup A, B, C, E and H have been implicated in swine enteric disease.
From the laboratory of Kyoungjin J. Yoon, DVM, PhD, Iowa State University.
| Product Type: | Antibody |
| Accession ID: | P14162 |
| Antigen: | Porcine rotavirus (PoRV) VP6 |
| Molecular Weight: | 44.6 kDa |
| Isotype: | IgG |
| Clonality: | Monoclonal |
| Clone Name: | 11H3 |
| Reactivity: | Porcine |
| Immunogen: | Purified recombinant PoRV VP6 subclass C |
| Species Immunized: | Mouse |
| Buffer: | 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide |
| Tested Applications: | ELISA, WB, IF, IHC |
| Storage: | -20C |
| Shipped: | Cold Packs |
Immunofluorescence Microscopy
Immunofluorescence microscopy of Sf9 cells inoculated with are combinant baculovirus expressing VP6 of PoRV B (A)or PoRV C (B) and baculovirus (C, negative control), respectively, after being stained with mAB10B1 and 11F3. Clones were specific positive to their targets.
Western Blot
Specificity of mAB 10B1 and 11F3 for PoRV B VP6 (A) And PoRV C VP6 (B), respectively as determined by WB
Immunohistochemistry
Immmunohistochemical staining of enterocytes (brown color) in an intestinal tissue from a CDCD piglet experimentally infected with PoRV C by mAB 11F3. The intestinal tissue also shows villous atrophy.
- Estes, M. K., and A.Z. Kapikian, 2007, Rotaviruses,in D.M.Knipe, and P.M. Howley, eds.,FieldsVirology, 5thEdition, Philadelphia: Lippincott-Raven and Wilkins Publishers,p.1917-1974.
- Matthijnssens, J., P. H.Otto, M. Ciarlet, U. Desselberger, M. Van Ranst, and R. Johne, 2012, VP6-sequence-based cut off values as a criterion for rotavirus species demarcation: Arch Virol,v.157,p.1177-82.
- Trojnar, E., P. Otto, and R. Johne, 2009, The first complete genome sequence of a chicken group A rotavirus indicates independent evolution of mammalian and avian strains: Virology,v.386,p.325-33.
- Cooke, S. J. , I . N . Clarke, R. B. Freitas, Y. B. Gabbay, and P.R. Lambden, 1992, The correct sequence of the porcine group C / Cowden rotavirus major innercapsid protein shows close homology with human isolates from Brazil and the U.K: Virology,v.190,p.531-7.
If you publish research with this product, please let us know so we can cite your paper.
