Anti-TXNDC8 (SPTRX-3) Antibody
This rabbit polyclonal antibody was generated against thioredoxin domain-containing protein 8 (TXNDC8, SPTRX-3) and recognizes human, mouse, and rat TXNDC8.
Highlights:
- Reacts with human, mouse, and rat TXNDC8
- Suitable for Western Blot, ELISA, Flow Cytometry, Immunohistochemistry, and Immunoflourescence applications
Thioredoxin domain-containing protein 8 (TXNDC8, SPTRX-3) may be required for post-translational modification of proteins required for acrosomal biogenesis. TXNDC8 may act by reducing disulfide bonds within the sperm. The protein is testis-specific, and is only expressed during spermiogenesis, prominently in the Golgi apparatus of pachytene spermatocytes and round and elongated spermatids, with a transient localization in the developing acrosome of round spermatids. TXNDC8 accumulates in superfluous cytoplasm of defective human spermatozoa.
From the laboratory of Peter Sutovsky, PhD, University of Missouri-Columbia.
This rabbit polyclonal antibody was generated against thioredoxin domain-containing protein 8 (TXNDC8, SPTRX-3) and recognizes human, mouse, and rat TXNDC8.
Highlights:
- Reacts with human, mouse, and rat TXNDC8
- Suitable for Western Blot, ELISA, Flow Cytometry, Immunohistochemistry, and Immunoflourescence applications
Thioredoxin domain-containing protein 8 (TXNDC8, SPTRX-3) may be required for post-translational modification of proteins required for acrosomal biogenesis. TXNDC8 may act by reducing disulfide bonds within the sperm. The protein is testis-specific, and is only expressed during spermiogenesis, prominently in the Golgi apparatus of pachytene spermatocytes and round and elongated spermatids, with a transient localization in the developing acrosome of round spermatids. TXNDC8 accumulates in superfluous cytoplasm of defective human spermatozoa.
From the laboratory of Peter Sutovsky, PhD, University of Missouri-Columbia.
| Product Type: | Antibody |
| Alternative Name(s): | Spermatid-specific thioredoxin-3 |
| Antigen: | TXNDC8, SPTRX-3, Thioredoxin-6 |
| Accession ID: | Q6A555 |
| Molecular Weight: | 20 kDa |
| Isotype: | IgG |
| Clonality: | Polyclonal |
| Reactivity: | Human, Mouse, Rat |
| Immunogen: | Mixture of peptides from sequences encoded within exon I [(NH2-) MVQIIKDTNEFKTFC (-COOH)] and exon V [(Ac-) VTLFSRIKRIIC (-COOH)] of murine TXNDC8 |
| Species Immunized: | Rabbit |
| Purification Method: | Immunogen affinity purification |
| Buffer: | affinity purified rabbit serum (pH 7.2, 0.05% sodium azide) |
| Tested Applications: | WB 1:2000, ELISA 1:200-1:2000, Flow cytometry 1:100, IHC 1:100, IF 1:100 |
| Concentration: | 1mg/mL |
| Comments: | Positive control: Human spermatozoa (IF), testicular tissue homogenate (WB, ELISA), or tissue sections (IHC) |
| Storage: | Upon delivery, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles |
| Shipped: | Cold Packs |
Representative experimental data for TXNDC8 (SPTRX-3) Antibody.
- Ahlering P, Sutovsky P, (2014) Biomarker-based flow-cytometric semen analysis for male infertility diagnostics and clinical decision making in ART. In: Screening for the Single Euploid Embryo - Molecular Genetics in Reproductive Medicine, Eric Scott Sills, Editor, Springer Science+Media LLC, p. 33-51.
- Buckman CL, Ozanon C, Qiu J, Sutovsky M, Carafa JA, Rawe VY, Manandhar G, Miranda-Vizuete A, Sutovsky P (2013) Semen levels of spermatid-specific thioredoxin-3 correlate with pregnancy rates in ART couples. PLoS ONE, e61000. doi: 10.1371.
- Jiménez A, Zu W, Rawe VY, Pelto-Huikko M, Flickinger CJ, Sutovsky P, Gustafsson J-A, Oko R and Antonio Miranda-Vizuete A. (2004) Spermatocyte/spermatid-specific thioredoxin-3, a novel Golgi apparatus-associated thioredoxin, is a specific marker of aberrant spermatogenesis. J. Biol. Chem. 279(33):34971-34982.
- Miranda-Vizuete A, Sadek CM, Jiménez A, Krause WJ, Sutovsky P and Oko R (2004) The mammalian testis-specific thioredoxin system. Antioxid Redox Signal. 6:25-40.
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