Desmosterol
Sterol intermediate in the cholesterol biosynthesis pathway. Reacts with the dienophile, 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD) to give products that have unique MS fragmentation patterns. This assay increases the sensitivity and shortens the analysis time. In addition, the availability of isotopic standards allows quantitation of the sterol.
From the laboratory of Ned A. Porter, PhD and Keri A. Tallman, PhD, Vanderbilt University.
Sterol intermediate in the cholesterol biosynthesis pathway. Reacts with the dienophile, 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD) to give products that have unique MS fragmentation patterns. This assay increases the sensitivity and shortens the analysis time. In addition, the availability of isotopic standards allows quantitation of the sterol.
From the laboratory of Ned A. Porter, PhD and Keri A. Tallman, PhD, Vanderbilt University.
Specifications
| Product Type: | Small Molecule |
| Name: | Desmosterol |
| Chemical Formula: | C27H44O |
| Source: | synthetic |
| Molecular Weight: | 384.65 |
| Format: | white powder |
| Purity: | >95%, NMR |
| Solubility: | CHCl3, THF, benzene |
| Spectral Information: | 1H, 13C NMR |
| Storage: | -20C |
| Shipped: | Ambient temperature |
Provider
From the laboratory of Ned A. Porter, PhD and Keri A. Tallman, PhD, Vanderbilt University.
References
- The Effect of Small Molecules on Sterol Homeostasis: Measuring 7-Dehydrocholesterol in Dhcr7-Deficient Neuro2a Cells and Human Fibroblasts. Korade, Z.; Kim, H.-Y. H.; Tallman, K. A.; Liu, W.; Koczok, K.; Balogh, I.; Xu, L.; Mirnics, K.; Porter, N. A. J. Medicinal Chemistry 2016, 59, 1102.
- A highly sensitive method for analysis of 7-dehydrocholesterol for the study of Smith-Lemli-Opitz syndrome. Liu, W.; Xu, L.; Lamberson, C.; Haas, D.; Korade, Z.; Porter, N. A. J. Lipid Research 2014, 55, 329.
- Korade Z, Genaro-Mattos TC, Tallman KA, Liu W, Garbett KA, Koczok K, Balogh I, Mirnics K, Porter NA. Vulnerability of DHCR7+/- mutation carriers to aripiprazole and trazodone exposure. J Lipid Res. 2017 Sep 28. pii: jlr.M079475. View Article
- Wages PA, Kim HH, Korade Z, Porter NA. Identification and Characterization of Prescription Drugs that Change Levels of the Cholesterol Precursors 7 Dehydrocholesterol and Desmosterol. View Article
- Genaro-Mattos TC, Allen LB, Anderson A, Tallman KA, Porter NA, Korade Z, Mirnics K. Maternal aripiprazole exposure interacts with 7-dehydrocholesterol reductase mutations and alters embryonic neurodevelopment. Mol Psychiatry. 2019 Apr;24(4):491-500. View Article
If you publish research with this product, please let us know so we can cite your paper.
