8-Dehydrocholesterol (d7 isotope)
Isotopically labeled sterol intermediate in the cholesterol biosynthesis pathway. Reacts with the dienophile, 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD) to give products that have unique MS fragmentation patterns. This assay increases the sensitivity and shortens the analysis time.
From the laboratory of Ned A. Porter, PhD and Keri A. Tallman, PhD, Vanderbilt University.
Isotopically labeled sterol intermediate in the cholesterol biosynthesis pathway. Reacts with the dienophile, 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD) to give products that have unique MS fragmentation patterns. This assay increases the sensitivity and shortens the analysis time.
From the laboratory of Ned A. Porter, PhD and Keri A. Tallman, PhD, Vanderbilt University.
Specifications
| Product Type: | Small Molecule |
| Name: | 8-Dehydrocholesterol-25,26,26,26,27,27,27-d7 |
| Chemical Formula: | C27H37D7O |
| Source: | synthetic |
| Molecular Weight: | 391.69 |
| Format: | white powder |
| Purity: | >95%, NMR |
| Solubility: | CHCl3, THF, benzene |
| Isotopic Purity: | 92% |
| Mass Shift: | m/z 7 |
| Comments: | susceptible to oxidation |
| Storage: | -80C |
| Shipped: | Dry ice |
Provider
From the laboratory of Ned A. Porter, PhD and Keri A. Tallman, PhD, Vanderbilt University.
References
- The Effect of Small Molecules on Sterol Homeostasis: Measuring 7-Dehydrocholesterol in Dhcr7-Deficient Neuro2a Cells and Human Fibroblasts. Korade, Z.; Kim, H.-Y. H.; Tallman, K. A.; Liu, W.; Koczok, K.; Balogh, I.; Xu, L.; Mirnics, K.; Porter, N. A. J. Medicinal Chemistry 2016, 59, 1102.
- A highly sensitive method for analysis of 7-dehydrocholesterol for the study of Smith-Lemli-Opitz syndrome. Liu, W.; Xu, L.; Lamberson, C.; Haas, D.; Korade, Z.; Porter, N. A. J. Lipid Research 2014, 55, 329.
- Kanuri B, Fong V, Ponny SR, Tallman KA, Ramachandra Rao S, Porter NA, Fliesler SJ, Patel SB. Generation and validation of a conditional knockout mouse model for the study of the Smith-Lemli-Opitz Syndrome. J Lipid Res. 2020 Nov 17:jlr.RA120001101. View article
- Korade Z, Allen LB, Anderson A, Tallman KA, Genaro-Mattos TC, Porter NA, Mirnics K. Trazodone effects on developing brain. Transl Psychiatry. 2021 Feb 1;11(1):85. View article
If you publish research with this product, please let us know so we can cite your paper.
