Cystic Fibrosis Human Bronchial Epithelial Cell Line (CF/T43)
An airway epithelial cell line (CF/T43) was developed by infecting cultured airway epithelial cells from patients with cystic fibrosis (CF) with the pZIPneoSV(X)1/SV40T retrovirus and selecting for G418 resistance and ion transport properties.
Highlights
- Display distinctive chloride secretory phenotypes
- Useful for testing candidate CF genes and evaluating molecular mechanisms responsible for the CF phenotype
Cystic fibrosis (CF) is a common generalized disorder of exocrine gland function which impairs clearance of secretions in a variety of organs. It is characterized by the triad of chronic bronchopulmonary disease (with recurrent respiratory infections), pancreatic insufficiency (which leads to malabsorption and growth retardation) and elevated sweat electrolytes.
From the laboratory of Anton Jetten, PhD, National Institute of Environmental Health Sciences/NIH.
An airway epithelial cell line (CF/T43) was developed by infecting cultured airway epithelial cells from patients with cystic fibrosis (CF) with the pZIPneoSV(X)1/SV40T retrovirus and selecting for G418 resistance and ion transport properties.
Highlights
- Display distinctive chloride secretory phenotypes
- Useful for testing candidate CF genes and evaluating molecular mechanisms responsible for the CF phenotype
Cystic fibrosis (CF) is a common generalized disorder of exocrine gland function which impairs clearance of secretions in a variety of organs. It is characterized by the triad of chronic bronchopulmonary disease (with recurrent respiratory infections), pancreatic insufficiency (which leads to malabsorption and growth retardation) and elevated sweat electrolytes.
From the laboratory of Anton Jetten, PhD, National Institute of Environmental Health Sciences/NIH.
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| Product Type: | Cell Line |
| Name: | CF/T43 |
| Cell Type: | Airway epithelial cells from cystic fibrosis patients |
| Accession ID: | CVCL_HA79 |
| Organism: | Human |
| Source: | Airways |
| Morphology: | Epithelioid |
| Biosafety Level: | BL2 |
| Growth Conditions: | Ham’s F12 (plus 1 mM glutamine, 14.3 mM Na-bicarbonate, 15 mM Hepes, pH 7.2), penicillin 100 U/ml, streptomycin sulfate 100 ug/ml, gentamycin 50 ug/ml, Insulin 10 ug/ml, hydrocortisone 1 uM, endothelial growth supplement (ECGS) 3.75 ug/ml, epidermal growth factor (EGF) 25 ng/ml, triiodothyronine (T3) 30 nM, transferrin 5 ug/ml, cholera toxin 10ng/ml |
| Subculturing: | Doubling time 24-28hrs; Trypsinize at 70-80% confluency and replate at 1:8-10 |
| Cryopreservation: | Growth medium plus 20% FBS, 7.5% DMSO |
| Storage: | Liquid nitrogen |
| Shipped: | Dry Ice |
- Jetten, A.M., Yankaskas, J.R., Stutts, M.J., Willumsen, N.J. and Boucher, R.C. Persistence of abnormal chloride conductance regulation in transformed cystic fibrosis airway epithelia. Science 244:1472-1475(1989)
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