This mouse IgG2a monoclonal antibody was raised against ricin A chain and is specific for Ricinus communis ricin toxin A chain.
Highlights:
Ricin is highly toxic to animal cells and to a lesser extent to plant cells. The A chain acts as a glycosidase that removes a specific adenine residue from an exposed loop of the 28S rRNA (A4324 in mammals), leading to rRNA breakage. As this loop is involved in elongation factor binding, modified ribosomes are catalytically inactive and unable to support protein synthesis. The A chain can inactivate a few thousand ribosomes per minute, faster than the cell can make new ones. Therefore a single A chain molecule can kill an animal cell. The B chain binds to beta-D-galactopyranoside moieties on cell surface glycoproteins and glycolipids and facilitates the entry into the cell of the A chain; B chains are also responsible for cell agglutination (Lectin activity).
From the laboratory of Seth H. Pincus, MD, Montana State University.
Product Type: | Antibody |
Accession ID: | P02879 |
Antigen: | Ricin toxin A chain |
Isotype: | IgG2a |
Clonality: | Monoclonal |
Clone Name: | RAC18 |
Reactivity: | Ricinus communis |
Immunogen: | Ricin A chain |
Species Immunized: | Mouse |
Epitope: | For epitope mapping, see: Maddaloni et al, J. Immunol 2004, 172:6221-28. |
Purification Method: | Protein G |
Buffer: | 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide |
Tested Applications: | Antigen capture ELISA for ricin toxin, immunofluorescence, 1-10µg/ml, Neutralization of ricin toxin in vitro and in vivo, effective concentrations dependent on ricin exposure |
Comments: | For validation of Ag-capture assay for ricin toxin, see: Simon et al, Toxins, 2015, 7:4967-86 |
Storage: | -20C |
Shipped: | Cold packs |
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