Marek's Disease Ovarian Cell Line (MDCC-UA04)
MDCC (Marek's disease cell culture) UA04 cells were established from a ovary-localized lymphoma using a recombinant Marek's disease virus (MDV), strain RB-1B, in which the US2 gene was disrupted by a CMV-gfp (S65T) expression cassette in which the splice donor site downstream of the GFP coding sequence was deleted.
Highlights:
- Expression of GFP is tightly controlled in these cells, with 2 - 5 % spontaneously expressing GFP
- Expression is induced during reactivation of the MDV from latency, therefore cells are ideal for studying the early events of reactivation
Marek's disease is a highly contagious viral neoplastic disease in chickens. Marek's disease is caused by an alphaherpesvirus known as 'Marek's disease virus' (MDV) or Gallid herpesvirus 2 (GaHV-2). The disease is characterized by the presence of T cell lymphoma as well as infiltration of nerves and organs by lymphocytes.
From the laboratory of Mark S. Parcells, PhD, University of Delaware.
MDCC (Marek's disease cell culture) UA04 cells were established from a ovary-localized lymphoma using a recombinant Marek's disease virus (MDV), strain RB-1B, in which the US2 gene was disrupted by a CMV-gfp (S65T) expression cassette in which the splice donor site downstream of the GFP coding sequence was deleted.
Highlights:
- Expression of GFP is tightly controlled in these cells, with 2 - 5 % spontaneously expressing GFP
- Expression is induced during reactivation of the MDV from latency, therefore cells are ideal for studying the early events of reactivation
Marek's disease is a highly contagious viral neoplastic disease in chickens. Marek's disease is caused by an alphaherpesvirus known as 'Marek's disease virus' (MDV) or Gallid herpesvirus 2 (GaHV-2). The disease is characterized by the presence of T cell lymphoma as well as infiltration of nerves and organs by lymphocytes.
From the laboratory of Mark S. Parcells, PhD, University of Delaware.
| Product Type: | Cell Line |
| Cell Type: | Chicken T-cells (CD4+, TCR2+, CD3lo, CD28lo, MHC-IIhi) |
| Morphology: | non-adherent, pleomorphic, lymphoblastoid |
| Source: | Ovarian Lymphoma |
| Organism: | Chicken, Gallus gallus, (SPF single-comb white leghorn) |
| Biosafety Level: | BSL-1 |
| Subculturing: | 1:5 to 1:10 every 2 - 3 days |
| Growth Conditions: | Established LM Hahn medium supplemented with 10% FBS,10% chicken serum, 10% tryptose-phosphate broth, 4 mM L-glutamine, 2 µM 2-mercaptoethanol, 1X Pen/Strept/Neo, 1X fungizone. Adapted to: RPM1-1640, 20% FBS, 4 mM L-glutamine, 1 X PSN, 1X fungizone |
| Cryopreservation: | 90% FBS/10% DMSO |
| Comments: | Grow at 41C, 5% CO2. When actively growing, double every 12 - 24 hrs. Grow at 5 x 105 to 2 x 107 cells/ml. |
| Storage: | Liquid nitrogen |
| Shipped: | Dry Ice |
- Dienglewicz, RL and MS Parcells. 1999. Establishment of a lymphoblastoid cell line using a mutant MDV containing a green fluorescent protein expression cassette. Acta Virol. 43:106-1112.
- Hunt, HD, B Lupiani, MM Miller, I Gimeno, LF Lee and MS. Parcells. 2001. Marek's disease virus down-regulates surface expression of MHC (B Complex) Class I (BF) glycoproteins during active but not latent infection of chicken cells. Virology 282:198-205.
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