Marek's Disease Ovarian Cell Line (MDCC-UD14)
MDCC (Marek's disease cell culture) UD14 cells were established from a kidney-localized lymphoma using the RB-1B strain of Marek's disease virus (MDV).
Marek's disease is a highly contagious viral neoplastic disease in chickens. Marek's disease is caused by an alphaherpesvirus known as 'Marek's disease virus' (MDV) or Gallid herpesvirus 2 (GaHV-2). The disease is characterized by the presence of T cell lymphoma as well as infiltration of nerves and organs by lymphocytes.
From the laboratory of Mark S. Parcells, PhD, University of Delaware.
MDCC (Marek's disease cell culture) UD14 cells were established from a kidney-localized lymphoma using the RB-1B strain of Marek's disease virus (MDV).
Marek's disease is a highly contagious viral neoplastic disease in chickens. Marek's disease is caused by an alphaherpesvirus known as 'Marek's disease virus' (MDV) or Gallid herpesvirus 2 (GaHV-2). The disease is characterized by the presence of T cell lymphoma as well as infiltration of nerves and organs by lymphocytes.
From the laboratory of Mark S. Parcells, PhD, University of Delaware.
Specifications
| Product Type: | Cell Line |
| Cell Type: | Chicken T-cells (CD4hi, TCR2hi, TCR3lo, CD3hi, CD28hi , CD29hi, CD30hi, CD44hi, CD45lo, CD164hi, MHC-Ihi, MHC-IIhi) |
| Source: | kidney-localized lymphoma |
| Organism: | Chicken, Gallus gallus, (SPF single-comb white leghorn) |
| Morphology: | non-adherent, pleomorphic, lymphoblastoid |
| Biosafety Level: | BSL-1 |
| Subculturing: | 1:5 to 1:10 every 2 - 3 days |
| Growth Conditions: | Established: LM Hahn medium supplemented with 10% FBS,10% chicken serum, 10% tryptose-phosphate broth, 4 mM L-glutamine, 2 µM 2-mercaptoethanol, 1X Pen/Strept/Neo, 1X fungizone. Adapted to: RPM1-1640, 10% FBS, 4 mM L-glutamine, 1 X PSN, 1X fungizone |
| Cryopreservation: | 90% FBS/10% DMSO |
| Comments: | Grow at 41C, 5% CO2. When actively growing, double every 12 - 24 hrs. Grow at 5 x 105 to 2 x 107 cells/ml. |
| Storage: | Liquid nitrogen |
| Shipped: | Dry Ice |
Provider
From the laboratory of Mark S. Parcells, PhD, University of Delaware.
References
- Parcells, MS, AS Anderson, and RW Morgan. 1995. Retention of Oncogenicity by a Marek’s Disease Virus Mutant Lacking Six Unique Short Region Genes. J. Virol. 69: 7888 - 7898.
- Parcells, MS, RL Dienglewicz, AS Anderson, and RW Morgan. 1999. Recombinant Marek's disease viruses (MDV)-derived lymphoblasoid cell lines: regulation of a marker gene in the context of the MDV genome. J. Virol. 73:1362-1373(1999).
- Parcells, MS and SC Burgess. 2008. Immunological aspects of Marek's disease virus (MDV)-induced lymphoma progression. Chapter 8 in Selected Aspects of Cancer Progression: Metastasis, Apoptosis and Immune Response. H.E. Kaiser and A. Nasir, Eds. Springer Science and Business Media. P.169 - 191.
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