This rat IgM monoclonal antibody was generated against polysaccharide and recognizes land plants pectic polysaccharide, rhamnogalacturonan-I (RG-I).
Highlights:
Generated subsequent to immunization with a pectic fraction. Recognizes a epitope associated with arabinans and can be generated by arabinofuranosidase action and the loss of arabinosyl residues. The binding of LM16 is sensitive to galactosidase action and the epitope may involve galactosyl residue(s) on RG backbones. Pectin is made up of a complex set of polysaccharides that are found in most primary cell walls and are particularly abundant in the non-woody parts of terrestrial plants. Pectin is a major component of the middle lamella, where it helps to bind cells together, but is also found in primary cell walls.
From the laboratory of Paul Knox, PhD, University of Leeds.
Product Type: | Antibody |
Antigen: | Pectic polysaccharide, Rhamnogalacturonan |
Isotype: | IgM |
Clonality: | Monoclonal |
Clone Name: | LM16 |
Reactivity: | Land plants |
Immunogen: | Polysaccharide |
Species Immunized: | Rat |
Epitope: | galactosylated RG-I |
Buffer: | Cell Culture Supernatant, 0.05% Sodium Azide |
Tested Applications: | IF (1:10), ELISA (1:10) |
Storage: | +4C (short term), -20C (long term) |
Shipped: | Ambient temperature |
Immunofluorescence
Tobacco stem
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