Trichoplusia ni Cell Line (Tni-FNL)
Trichoplusia ni cell line derived from BTI-Tn-5B1-4, adapted for suspension growth and serum-free growth.
Highlights
- Used for protein expression by baculovirus infection
- Can grow efficiently at 21 or 27 C in adherent or suspension conditions
The baculovirus expression vector system (BEVS) has proven to be a powerful technology for expression of recombinant proteins in eukaryotic cells. The BEVS technology has many advantages that include the capacity for large DNA insertions, the capacity for simultaneous expression of multiple genes, high expression levels for many proteins, and posttranslational modifications that often resemble those found in mammalian cells.
From the laboratory of Dominic Esposito, PhD, National Cancer Institute/NIH.
Trichoplusia ni cell line derived from BTI-Tn-5B1-4, adapted for suspension growth and serum-free growth.
Highlights
- Used for protein expression by baculovirus infection
- Can grow efficiently at 21 or 27 C in adherent or suspension conditions
The baculovirus expression vector system (BEVS) has proven to be a powerful technology for expression of recombinant proteins in eukaryotic cells. The BEVS technology has many advantages that include the capacity for large DNA insertions, the capacity for simultaneous expression of multiple genes, high expression levels for many proteins, and posttranslational modifications that often resemble those found in mammalian cells.
From the laboratory of Dominic Esposito, PhD, National Cancer Institute/NIH.
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| Product Type: | Cell Line |
| Name: | Tni-FNL |
| Cell Type: | Insect |
| Accession ID: | CVCL_RY32 |
| Organism: | Trichoplusia ni |
| Source: | Ovary |
| Morphology: | Rounded spheres |
| Biosafety Level: | BSL2 |
| Subculturing: | 5 x 105 cells/ml for 4 day growth, 6 x 105 cells/ml for 3 day growth |
| Growth Conditions: |
Sf-900 III Serum Free Media; 21 hour doubling time, Max Growth 5 x 106 cells/ml. Temperature: 27C. Growth Conditions: Shake culture at 125 RPM for shaker board with 2" orbit or 185 RPM with a 1" orbit in Thomson Instrument Optimum Growth Flasks. |
| Cryopreservation: | 90% Growth Media; 10% DMSO; 0.15M Trehalose |
| Comments: | Use of 10 units/ml of Heparin can be used on highly aggregated cultures to disperse aggregation |
| Storage: | Liquid nitrogen |
| Shipped: | Dry Ice |
- Mehalko JL, Esposito D. Engineering the transposition-based baculovirus expression vector system for higher efficiency protein production from insect cells. J Biotechnol. 2016 Nov 20;238:1-8.
- Gillette WK, Esposito D, Abreu Blanco M, Alexander P, Bindu L, Bittner C, Chertov O, Frank PH, Grose C, Jones JE, Meng Z, Perkins S, Van Q, Ghirlando R, Fivash M, Nissley DV, McCormick F, Holderfield M, Stephen AG. Farnesylated and methylated KRAS4b: high yield production of protein suitable for biophysical studies of prenylated protein-lipid interactions. Sci Rep. 2015 Nov 2;5:15916.
- Gillette W, Frank P, Perkins S, Drew M, Grose C, Esposito D. Production of Farnesylated and Methylated Proteins in an Engineered Insect Cell System. Methods Mol Biol. 2019;2009:259-277. View Article
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