BJAB cells expressing green fluorescence protein (GFP)-B-lymphocyte antigen CD20.
Highlights:
CD20, or B-lymphocyte antigen CD20, is a cell-surface protein expressed in most immune cells arising from the B-cell lineage and plays a role in cellular activation and proliferation. Importantly, CD20 is a recognized biomarker for a number of human disorders, including several autoimmune diseases and B-cell malignancies. BJAB cells come from a patient with Burkitt lymphoma, which is a form of non-Hodgkin's lymphoma in which cancer starts in immune cells called B-cells. GFP was fused to the amino terminus of CD20 by inserting human CD20 cDNA into the cloning site of the pEGFP expression vector using XhoI/SacII. Stable transfectants of BJAB cells expressing green fluorescence protein (GFP)-CD20 were generated by transfection and selection using G418 and cell sorting.
From the laboratory of Julie P. Deans, PhD, University of Calgary.
Product Type: | Cell Line |
Cell Type: | Lymphoid |
Accession ID: | P11836 |
Source: | Patient with Burkitt's lymphoma |
Organism: | Human |
Morphology: | Typical lymphoid |
Biosafety Level: | BSL 2 |
Growth Conditions: | RPMI 10% FBS, with geneticin 0.4 mg/mL if needed |
Cryopreservation: | 10% DMSO and 90% FBS |
Comments: | GFP was fused to the amino terminus of CD20 by inserting human CD20 cDNA into the cloning site of the pEGFP expression vector using XhoI/SacII. Stable transfectants of BJAB cells expressing green fluorescence protein (GFP)-CD20 were generated by transfection and selection using G418 and cell sorting. |
Storage: | LN2 |
Shipped: | Dry Ice |
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