MS4A4A-GFP Expressing 300-19 Cell Line
300-19 cells expressing green fluorescence protein (GFP)-Membrane-Spanning 4-Domains, Subfamily A, Member 4A (MS4A4A).
Highlights:
- Cells express GFP and MS4A4A
MS4A (membrane-spanning 4-domain family, subfamily A) is a large family of proteins that includes at least 26 members in mouse and humans. Flanked by amino- and carboxyl- cytoplasmic regions, MS4A family members contain four highly conserved transmembrane domains connected by short extracellular and cytoplasmic loops. CD20, the most well-known MS4A family member, is a B-cell-specific molecule associated with the B cell antigen receptor and functions in receptor-activated calcium influx. Several other MS4A family members are likely to be components of oligomeric cell surface complexes involved in signal transduction in diverse cell lineages.
From the laboratory of Julie P. Deans, PhD, University of Calgary.
300-19 cells expressing green fluorescence protein (GFP)-Membrane-Spanning 4-Domains, Subfamily A, Member 4A (MS4A4A).
Highlights:
- Cells express GFP and MS4A4A
MS4A (membrane-spanning 4-domain family, subfamily A) is a large family of proteins that includes at least 26 members in mouse and humans. Flanked by amino- and carboxyl- cytoplasmic regions, MS4A family members contain four highly conserved transmembrane domains connected by short extracellular and cytoplasmic loops. CD20, the most well-known MS4A family member, is a B-cell-specific molecule associated with the B cell antigen receptor and functions in receptor-activated calcium influx. Several other MS4A family members are likely to be components of oligomeric cell surface complexes involved in signal transduction in diverse cell lineages.
From the laboratory of Julie P. Deans, PhD, University of Calgary.
| Product Type: | Cell Line |
| Cell Type: | Pre-B |
| Accession ID: | Q96JQ5, CVCL_VD90 |
| Morphology: | Typical lymphoid |
| Organism: | Mouse |
| Source: | Mouse Bone Marrow |
| Biosafety Level: | BSL 2 |
| Growth Conditions: | RPMI 10% FBS |
| Cryopreservation: | 10% DMSO and 90% FBS |
| Comments: | Cloning of the human MS4A4A coding sequence and insertion into the pEGFP-C1 vector (Clontech, Mountain View, CA) was described in Front. Immunol. 2013; 4. Construct expressed by transfection with selection using G418. |
| Storage: | LN2 |
| Shipped: | Dry Ice |
- Zuccolo, J., Deng, L., Unruh, T.L., Sanyal, R., Bau, J.A., Storek, J., Demetrick, D.J., Luider, J.M., Auer-Grzesiak, I.A., Mansoor, A. and Deans, J.P. Expression of MS4A and TMEM176 genes in human B lymphocytes. Front. Immunol. 4: article 195 (2013)
- Sanyal R, Polyak MJ, Zuccolo J, Puri M, Deng L, Roberts L, Zuba A, Storek J, Luider JM, Sundberg EM, Mansoor A, Baigorri E, Chu MP, Belch AR, Pilarski LM, Deans JP. MS4A4A: a novel cell surface marker for M2 macrophages and plasma cells. Immunol Cell Biol. 2017 Aug;95(7):611-619.
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