Anti-Etv2 Antibody
This rabbit polyclonal antibody was generated against recombinant protein and is specific for zebrafish ETS Variant 2 (Etv2).
Highlights:
- Reacts with zebrafish Etv2
- Binds to the N-terminal half of Etv2
- Suitable for Western Blot and Whole Mount Immunostaining applications
Etv2 also known as Ets-related protein 71 is an endothelial-specific ETS transcription factor that is essential for vascular differentiation and morphogenesis in vertebrates.
From the laboratory of Nathan D. Lawson, PhD, University of Massachusetts Medical School.
This rabbit polyclonal antibody was generated against recombinant protein and is specific for zebrafish ETS Variant 2 (Etv2).
Highlights:
- Reacts with zebrafish Etv2
- Binds to the N-terminal half of Etv2
- Suitable for Western Blot and Whole Mount Immunostaining applications
Etv2 also known as Ets-related protein 71 is an endothelial-specific ETS transcription factor that is essential for vascular differentiation and morphogenesis in vertebrates.
From the laboratory of Nathan D. Lawson, PhD, University of Massachusetts Medical School.
| Product Type: | Antibody |
| Antigen: | Etv2 |
| Accession ID: | O00321, NP_001032452 |
| Molecular Weight: | 41 kDa |
| Clonality: | Polyclonal |
| Reactivity: | Zebrafish |
| Immunogen: | Recombinant protein |
| Species Immunized: | Rabbit |
| Epitope: | N-terminal half of Etv2 |
| Buffer: | Polyclonal serum with 0.02% sodium azide |
| Tested Applications: | WB (1:5000), Whole mount immunostaining (1:500) |
| Comments: | Recommend aliquotting into small working volumes after initial thaw and store these at -80°C |
| Storage: | -80C |
| Shipped: | Cold Packs (Domestic, Overnight); Dry Ice (International) |
Immunohistochemistry
Whole mount immunohistochemistry, using 12 or 17 hours post fertilization wild type zebrafish embryos (2%PFA fixed). Primary stain: anti-Etv2 rabbit polyclonal antibody (1:500 dilution). Secondary stain: Invitrogen Goat anti-rabbit Alexa fluor 488 (Cat#:A111034); [1:1,000 Dilution].
Western Blot
Western blot of HEK293T cell lysates cotransfected with a 6xc-Myc tagged version of Etv2 and Enhanced green fluorescent protein (EGFP). Lysates where run on a TRIS-Glycine gel and transfered to PDVF membrane. The blot was cut, blocked with 5% dried milk and probed individually with a 1:5,000 dilution of anti-Etv2 polyclonal antibody, 1:10,000 dilution of Sigma anti-c-Myc monoclonal antibody(clone: 9E10), or 1:5,000 dilution of Invitrogen anti-GFP rabbit polyclonal (Cat#:A11122). A 1:20,000 dilution of species specific HRP conjugated secondary was used for visualization.
Adapted from: Moore JC et al., Dev Biol. 2013 Dec 1;384(1):128-40.
- Moore JC, Sheppard-Tindell S, Shestopalov IA, Yamazoe S, Chen JK, Lawson ND. Post-transcriptional mechanisms contribute to Etv2 repression during vascular development. Dev Biol. 2013 Dec 1;384(1):128-40.
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