Anti-SARS Non structural protein 8 (NSP8) [5A10] Antibody
![Anti-SARS Non structural protein 8 (NSP8) [5A10] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1745_2001202001265917440.jpg "Anti-SARS Non structural protein 8 (NSP8) [5A10] Antibody")
This mouse IgG1 monoclonal antibody was raised against recombinant GST-NSP8 fusion protein and is specific for the non-structural protein 8 of SARS-Coronavirus (CoV).
Highlights:
- Reacts with the non-structural protein 8 of SARS-CoV
- Suitable for Western Blot and Immunofluorscence applications
Like other known coronaviruses, SARS-CoV is an enveloped, plus-strand RNA virus that features the largest RNA genomes currently known. SARS-CoV replicase polyproteins are processed by virus-encoded proteinases, and two proteinases have been identified: a 3C-like proteinase (3CLpro) and a papain-like proteinase (PLP). The processed end products of pp1a are designated nonstructural proteins (nsp) 1 to nsp11 and those of pp1ab are designated nsp1 to nsp16. Cleavage by the viral main protease, 3CLpro results in generating the nsp8 protein which is currently of undesignated function. The nsp8 protein has been indicated to associate with several other nsps and to colocalize with these nsps in cytoplasmic complexes that are important for viral RNA synthesis.
From the laboratory of A*STAR Institute of Molecular and Cell Biology (IMCB) Monoclonal Antibody Unit.
This mouse IgG1 monoclonal antibody was raised against recombinant GST-NSP8 fusion protein and is specific for the non-structural protein 8 of SARS-Coronavirus (CoV).
Highlights:
- Reacts with the non-structural protein 8 of SARS-CoV
- Suitable for Western Blot and Immunofluorscence applications
Like other known coronaviruses, SARS-CoV is an enveloped, plus-strand RNA virus that features the largest RNA genomes currently known. SARS-CoV replicase polyproteins are processed by virus-encoded proteinases, and two proteinases have been identified: a 3C-like proteinase (3CLpro) and a papain-like proteinase (PLP). The processed end products of pp1a are designated nonstructural proteins (nsp) 1 to nsp11 and those of pp1ab are designated nsp1 to nsp16. Cleavage by the viral main protease, 3CLpro results in generating the nsp8 protein which is currently of undesignated function. The nsp8 protein has been indicated to associate with several other nsps and to colocalize with these nsps in cytoplasmic complexes that are important for viral RNA synthesis.
From the laboratory of A*STAR Institute of Molecular and Cell Biology (IMCB) Monoclonal Antibody Unit.
| Product Type: | Antibody |
| Antigen: | Non-structural protein 8 of SARS-coronavirus |
| Accession ID: | A0A6B9VTP3 |
| Molecular Weight: | 22 kDa |
| Isotype: | IgG1 |
| Clonality: | Monoclonal |
| Clone Name: | 5A10 |
| Reactivity: | SARS-CoV |
| Immunogen: | Recombinant GST-NSP8 fusion protein |
| Species Immunized: | Mouse |
| Buffer: | Cell culture supernatant |
| Tested Applications: | WB, IF |
| Storage: | 80C |
| Shipped: | Cold Packs |
Western Blot
Supernatant from 5A10 hybridoma used as primary antibody.
- Meng B, Lui YW, Meng S, Cao C, Hu Y. Identification of Effective siRNA Blocking the Expression of SARS Viral Envelope E and RDRP Genes. Mol Biotechnol. 2006 Jun; 33(2):141 -8.
- Cheng A, Zhang W, Xie Y, Jiang W, Arnold E, Sarafianos SG, Ding J. Expression, purification, and haracterization of SARS coronavirus RNA polymerase. Virology. 2005 May; 335(2):165 -176.
- Sugiyama T, Cam H, Verdel A, Moazed D, Grewal SI. RNA-dependent RNA polymerase is an essential component of a self-enforcing loop coupling heterochromatin assembly to siRNA production. Proc Natl Acad Sci U S A 2005 Jan; 102(1):152-7.
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