Anti-Antiproliferative Factor (Gal-GalNAc-TVPAAVVVAC) [1G9-4-20] Antibody

Anti-Antiproliferative Factor (Gal-GalNAc-TVPAAVVVAC) [1G9-4-20] Antibody

This mouse monoclonal antibody was generated against a cysteinylated two-sugar antiproliferative factor (APF) derivative (Gal-GalNAc-TVPAAVVVAC) and recognizes two-sugar APF derivitives.

Antiproliferative factor (APF) is a low molecular weight, heat stable sialoglycopeptide that containsthe transmembrane segment of Frizzled 8. It is made and secreted by bladder epithelial cells of patients with acondition known as interstitial cystitis (IC) which causes chronic pain due to ulcers, hemorrhaging, and thinning of the bladder epithelium. APF contributes to the pathology of IC by inhibiting bladder cell proliferation, and was also shown to regulate proliferation of bladder carcinoma cells. Recently, CKAP4/p63 was identified as a receptor for APF, but how its activity is mediated remains unknown.

From the laboratory of Susan K. Keay, MD, PhD, Department of Veterans Affairs.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EVA001
Anti-Antiproliferative Factor (Gal-GalNAc-TVPAAVVVAC) [1G9-4-20] Antibody
100ug (1.54mg/mL) In stock
Regular Price:$345.00
On Sale:
Specifications

Product Type: Antibody
Antigen: Gal-GalNAc-TVPAAVVVAC
Accession ID: Q9H461
Molecular Weight: 1378 Da
Clonality: Monoclonal
Clone Name: 1G9-4-20
Immunogen: KLH-conjugated glycosylated peptide
Species Immunized: Mouse
Purification Method: Protein A
Method Used to Determine Concentration: OD280
Buffer: PBS, pH 7.4
Tested Applications: APF bioassay/dot blot
Concentration: 1.54mg/mL
Storage: -80C
Shipped: Cold Packs (Domestic, Overnight); Dry Ice (International)

Provider
From the laboratory of Susan K. Keay, MD, PhD, Department of Veterans Affairs.
References
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  2. Keay SK, Zhang CO. Abnormal Akt signalling in bladder epithelial cell explants from patients with interstitial cystitis/bladder pain syndrome can be induced by antiproliferative factor treatment of normal bladder cells. BJU Int. 2016 Jul;118(1):161-72. doi: 10.1111/bju.13457. Epub 2016 Mar 29. PubMed PMID: 26919663.
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  17. Erickson DR, Tomaszewski JE, Kunselman AR, Stetter CM, Peters KM, Rovner ES, Demers LM, Wheeler MA, Keay SK. Urine markers do not predict biopsy findings or presence of bladder ulcers in interstitial cystitis/painful bladder syndrome. J Urol. 2008 May;179(5):1850-6. doi: 10.1016/j.juro.2008.01.047. Epub 2008 Mar 18. PubMed PMID: 18353383; PubMed Central PMCID: PMC2705752.
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  27. Keay S, Seillier-Moiseiwitsch F, Zhang CO, Chai TC, Zhang J. Changes in human bladder epithelial cell gene expression associated with interstitial cystitis or antiproliferative factor treatment. Physiol Genomics. 2003 Jul 7;14(2):107-15. PubMed PMID: 12847144.
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  29. u Zhang CO, Li ZL, Shoenfelt JL, Kong CZ, Chai TC, Erickson DR, Peters KM, Rovner ES, Keay S. Comparison of APF activity and epithelial growth factor levels in urine from Chinese, African-American, and white American patients with interstitial cystitis. Urology. 2003 May;61(5):897-901. PubMed PMID: 12735999.
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  31. Chai TC, Zhang C, Warren JW, Keay S. Percutaneous sacral third nerve root neurostimulation improves symptoms and normalizes urinary HB-EGF levels and antiproliferative activity in patients with interstitial cystitis. Urology. 2000 May;55(5):643-6. PubMed PMID: 10792070.

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