Anti-Antiproliferative Factor (Gal-GalNAc-TVPAAVVVAC) [1G9-4-27] Antibody

Anti-Antiproliferative Factor (Gal-GalNAc-TVPAAVVVAC) [1G9-4-27] Antibody

This mouse monoclonal antibody was generated against a cysteinylated two-sugar antiproliferative factor (APF) derivative (Gal-GalNAc-TVPAAVVVAC) and recognizes two-sugar APF derivitives.

Antiproliferative factor (APF) is a low molecular weight, heat stable sialoglycopeptide that containsthe transmembrane segment of Frizzled 8. It is made and secreted by bladder epithelial cells of patients with acondition known as interstitial cystitis (IC) which causes chronic pain due to ulcers, hemorrhaging, and thinning of the bladder epithelium. APF contributes to the pathology of IC by inhibiting bladder cell proliferation, and was also shown to regulate proliferation of bladder carcinoma cells. Recently, CKAP4/p63 was identified as a receptor for APF, but how its activity is mediated remains unknown.

From the laboratory of Susan K. Keay, MD, PhD, Department of Veterans Affairs.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EVA006
Anti-Antiproliferative Factor (Gal-GalNAc-TVPAAVVVAC) [1G9-4-27] Antibody
100ug (4.15mg/mL) In stock
Regular Price:$345.00
On Sale:
Specifications

Product Type: Antibody
Antigen: Gal-GalNAc-TVPAAVVVAC
Accession ID: Q9H461
Molecular Weight: 1378 Da
Clonality: Monoclonal
Clone Name: 1G9-4-27
Immunogen: KLH-conjugated glycosylated peptide
Species Immunized: Mouse
Purification Method: Protein A
Method Used to Determine Concentration: OD280
Buffer: PBS, pH 7.4
Tested Applications: APF bioassay/dot blot
Concentration: 4.15mg/mL
Storage: -80C
Shipped: Cold Packs (Domestic, Overnight); Dry Ice (International)

Provider
From the laboratory of Susan K. Keay, MD, PhD, Department of Veterans Affairs.
References
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  2. Keay SK, Zhang CO. Abnormal Akt signalling in bladder epithelial cell explants from patients with interstitial cystitis/bladder pain syndrome can be induced by antiproliferative factor treatment of normal bladder cells. BJU Int. 2016 Jul;118(1):161-72. doi: 10.1111/bju.13457. Epub 2016 Mar 29. PubMed PMID: 26919663.
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  12. Sun Y, Keay S, Lehrfeld TJ, Chai TC. Changes in adenosine triphosphate-stimulated ATP release suggest association between cytokine and purinergic signaling in bladder urothelial cells. Urology. 2009 Nov;74(5):1163-8. doi: 10.1016/j.urology.2009.02.066. Epub 2009 Jul 22. PubMed PMID: 19628257; PubMed Central PMCID: PMC2777753.
  13. Planey SL, Keay SK, Zhang CO, Zacharias DA. Palmitoylation of cytoskeleton associated protein 4 by DHHC2 regulates antiproliferative factor-mediated signaling. Mol Biol Cell. 2009 Mar;20(5):1454-63. doi: 10.1091/mbc.E08-08-0849. Epub 2009 Jan 14. PubMed PMID: 19144824; PubMed Central PMCID: PMC2649263.
  14. Kim J, Keay SK, Freeman MR. Heparin-binding epidermal growth factor-like growth factor functionally antagonizes interstitial cystitis antiproliferative factor via mitogen-activated protein kinase pathway activation. BJU Int. 2009 Feb;103(4):541-6. doi: 10.1111/j.1464-410X.2008.08097.x. Epub 2008 Oct 16. PubMed PMID: 18990151; PubMed Central PMCID: PMC4000709.
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  17. Erickson DR, Tomaszewski JE, Kunselman AR, Stetter CM, Peters KM, Rovner ES, Demers LM, Wheeler MA, Keay SK. Urine markers do not predict biopsy findings or presence of bladder ulcers in interstitial cystitis/painful bladder syndrome. J Urol. 2008 May;179(5):1850-6. doi: 10.1016/j.juro.2008.01.047. Epub 2008 Mar 18. PubMed PMID: 18353383; PubMed Central PMCID: PMC2705752.
  18. Keay S, Reeder JE, Koch K, Zhang CO, Grkovic D, Peters K, Zhang Y, Kusek JW, Nyberg LM, Payne CK, Propert KJ. Prospective evaluation of candidate urine and cell markers in patients with interstitial cystitis enrolled in a randomized clinical trial of Bacillus Calmette Guerin (BCG). World J Urol. 2007 Oct;25(5):499-504. Epub 2007 Aug 13. PubMed PMID: 17694391.
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  26. Keay S, Zhang CO, Chai T, Warren J, Koch K, Grkovic D, Colville H, Alexander R. Antiproliferative factor, heparin-binding epidermal growth factor-like growth factor, and epidermal growth factor in men with interstitial cystitis versus chronic pelvic pain syndrome. Urology. 2004 Jan;63(1):22-6. PubMed PMID: 14751340.
  27. Keay S, Seillier-Moiseiwitsch F, Zhang CO, Chai TC, Zhang J. Changes in human bladder epithelial cell gene expression associated with interstitial cystitis or antiproliferative factor treatment. Physiol Genomics. 2003 Jul 7;14(2):107-15. PubMed PMID: 12847144.
  28. Keay S, Zhang CO, Shoenfelt JL, Chai TC. Decreased in vitro proliferation of bladder epithelial cells from patients with interstitial cystitis. Urology. 2003 Jun;61(6):1278-84. PubMed PMID: 12809929.
  29. u Zhang CO, Li ZL, Shoenfelt JL, Kong CZ, Chai TC, Erickson DR, Peters KM, Rovner ES, Keay S. Comparison of APF activity and epithelial growth factor levels in urine from Chinese, African-American, and white American patients with interstitial cystitis. Urology. 2003 May;61(5):897-901. PubMed PMID: 12735999.
  30. Keay SK, Zhang CO, Shoenfelt J, Erickson DR, Whitmore K, Warren JW, Marvel R, Chai T. Sensitivity and specificity of antiproliferative factor, heparin-binding epidermal growth factor-like growth factor, and epidermal growth factor as urine markers for interstitial cystitis. Urology. 2001 Jun;57(6 Suppl 1):9-14. PubMed PMID: 11378043.
  31. Chai TC, Zhang C, Warren JW, Keay S. Percutaneous sacral third nerve root neurostimulation improves symptoms and normalizes urinary HB-EGF levels and antiproliferative activity in patients with interstitial cystitis. Urology. 2000 May;55(5):643-6. PubMed PMID: 10792070.

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