Anti-Cytokeratin 8 [1E8] Antibody
This IgG2b mouse antibody was generated against the C-terminus of human Cytokeratin 8 (amino acids 472-483) and is reactive against human Cytokeratin 8.
Cytokeratin 8 is one member of a diverse group of proteins called intermediate filament proteins. These proteins are often expressed in pairs. Cytokeratin 8 pairs with Cytokeratin 18 to form a component of the cytoskeleton in simple epithelial cells. It is present on the surface of intact hepatocytes, hepatocellular carcinoma cells, and breast cancer cells. This monoclonal antibody is specific to Cytokeratin 8 and can therefore identify cells of these types.
From the laboratory of Steven L. Gonias, MD, PhD, University of Virginia.
Part of The Investigator's Annexe program.
This IgG2b mouse antibody was generated against the C-terminus of human Cytokeratin 8 (amino acids 472-483) and is reactive against human Cytokeratin 8.
Cytokeratin 8 is one member of a diverse group of proteins called intermediate filament proteins. These proteins are often expressed in pairs. Cytokeratin 8 pairs with Cytokeratin 18 to form a component of the cytoskeleton in simple epithelial cells. It is present on the surface of intact hepatocytes, hepatocellular carcinoma cells, and breast cancer cells. This monoclonal antibody is specific to Cytokeratin 8 and can therefore identify cells of these types.
From the laboratory of Steven L. Gonias, MD, PhD, University of Virginia.
Part of The Investigator's Annexe program.
| Product Type: | Antibody |
| Antigen: | cytokeratin 8 |
| Accession ID: | P05787 |
| Isotype: | IgG2b |
| Clone Name: | 1E8 |
| Reactivity: | Human |
| Species Immunized: | Mouse |
| Epitope: | C-terminus of human Cytokeratin 8 (amino acids 472-483) |
| Purification Method: | Protein A/G |
| Buffer: | PBS, 0.05% (w/v) Sodium Azide |
| Tested Applications: | WB, IF, IP |
| Storage: | -20C |
| Shipped: | Cold packs |
CK 8 specificity of antibody 1E8.
BT20 whole cell lysate (100 μg) was subjected to Western blot analysis using the well characterized CK 8-specific antibody, PCK-26, and the newly generated antibody, 1E8. Molecular mass standards (in-kDa) are shown.
Adapted from: Hembrough TA, Li L, Gonias SL. Cell-surface Cytokeratin 8 Is the Major Plasminogen Receptor on Breast Cancer Cells and Is Required for the Accelerated Activation of Cell-associated Plasminogen by Tissue-type Plasminogen Activator. J Biol Chem. 271(41): 25684-91 (1996).
- Hembrough TA, Li L, Gonias SL. Cell-surface Cytokeratin 8 Is the Major Plasminogen Receptor on Breast Cancer Cells and Is Required for the Accelerated Activation of Cell-associated Plasminogen by Tissue-type Plasminogen Activator. J Biol Chem. 271(41): 25684-91 (1996).
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