These rabbit polyclonal antibodies were generated against BSA conjugated ecdysteroids E and 20E and, in combination with BLG-conjugated ecdysteroids E and 20E, allow for a fast, accurate and low-cost means to measure titers for ecdysone (E) and 20-hydroxyecdysone (20E).
Highlights:
Ecdysteroid hormones regulate several aspects of insect development and reproduction. The predominant ecdysteroids produced by insects including mosquitoes are ecdysone (E) and 20-hydroxyecdysone (20E). The ability to measure E and 20E titers is essential for many studies. These antibodies BLG-conjugated antigens allow for a fast, accurate and low-cost means to measures titers for ecdysone (E) and 20-hydroxyecdysone (20E).
From the laboratory of Mark R. Brown, PhD and Michael R. Strand, PhD, University of Georgia.
Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
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Product Type: | Antibody |
Accession ID: | P34021 |
Clonality: | Polyclonal |
Specificity: |
EAB25: specific for 20-Hydroxyecdysone (20E) EAB27: recognizes both ecdysone (E) and 20-hydroxyecdysone (20E), and somewhat makisterone A |
Species Immunized: | Rabbit |
Buffer: | Whole serum for antibodies, PBS with 0.02% sodium azide for Ecdysteroid-BLG conjugates |
Tested Applications: | Enzymelinked immunoassay (EIA) (Protocol); Not suitable for immunocytochemistry or western blots |
Comments: | Antisera EAB25 and EAB27 were produced to ecdysone (E) conjugated to bovine serum albumin (BSA) or to 20-Hydroxyecdysone (20E) conjugated to BSA, so they should not be used in an enzyme immunoassay with BSA. |
Storage: | -80C |
Shipped: | Cold Packs (Domestic, Overnight); Dry Ice (International) |
Table of Antisera Properties
Antisera (antigen) | Affinity | Final use conc. | Detection Range (pg) |
EAB25 (20E-BSA) | 20E | 1:40,000 | 20E: 30-2,000 |
EAB27 (E-BSA) | E, 20E | 1:3,000 | E/20E: 4-500 |
Adapted from: McKinney DA, et al. (2017) Gen Comp Endocrinol. 253:60-69.
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