Anti-Ecdysteroid E/20E Antibodies and Antigens
These rabbit polyclonal antibodies were generated against BSA conjugated ecdysteroids E and 20E and, in combination with BLG-conjugated ecdysteroids E and 20E, allow for a fast, accurate and low-cost means to measure titers for ecdysone (E) and 20-hydroxyecdysone (20E).
Highlights:
- EAB25 is specific for 20-Hydroxyecdysone (20E) and should be purchased with the 20E-BLG conjugate, which is used to coat the assay plate
- EAB27 recognizes both ecdysone (E) and 20-hydroxyecdysone (20E), and somewhat makisterone A and can be used with either E-BLG or 20E-BLG to coat plates
- Neither antiserum recognizes 7-dehydrocholesterol
- Ecdysone (E)-Bovine beta lactoglobulin (BLG) or 20-Hydroxyecdysone (20E)-BLG conjugates, for use with enzyme immunoassay
- Suitable for enzyme immunoassay (See: Protocol); Not suitable for immunocytochemistry or western blots
Ecdysteroid hormones regulate several aspects of insect development and reproduction. The predominant ecdysteroids produced by insects including mosquitoes are ecdysone (E) and 20-hydroxyecdysone (20E). The ability to measure E and 20E titers is essential for many studies. These antibodies BLG-conjugated antigens allow for a fast, accurate and low-cost means to measures titers for ecdysone (E) and 20-hydroxyecdysone (20E).
From the laboratory of Mark R. Brown, PhD and Michael R. Strand, PhD, University of Georgia.
These rabbit polyclonal antibodies were generated against BSA conjugated ecdysteroids E and 20E and, in combination with BLG-conjugated ecdysteroids E and 20E, allow for a fast, accurate and low-cost means to measure titers for ecdysone (E) and 20-hydroxyecdysone (20E).
Highlights:
- EAB25 is specific for 20-Hydroxyecdysone (20E) and should be purchased with the 20E-BLG conjugate, which is used to coat the assay plate
- EAB27 recognizes both ecdysone (E) and 20-hydroxyecdysone (20E), and somewhat makisterone A and can be used with either E-BLG or 20E-BLG to coat plates
- Neither antiserum recognizes 7-dehydrocholesterol
- Ecdysone (E)-Bovine beta lactoglobulin (BLG) or 20-Hydroxyecdysone (20E)-BLG conjugates, for use with enzyme immunoassay
- Suitable for enzyme immunoassay (See: Protocol); Not suitable for immunocytochemistry or western blots
Ecdysteroid hormones regulate several aspects of insect development and reproduction. The predominant ecdysteroids produced by insects including mosquitoes are ecdysone (E) and 20-hydroxyecdysone (20E). The ability to measure E and 20E titers is essential for many studies. These antibodies BLG-conjugated antigens allow for a fast, accurate and low-cost means to measures titers for ecdysone (E) and 20-hydroxyecdysone (20E).
From the laboratory of Mark R. Brown, PhD and Michael R. Strand, PhD, University of Georgia.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Antibody |
| Clonality: | Polyclonal |
| Species Immunized: | Rabbit |
| Buffer: | Whole serum |
| Storage: | -80C |
| Shipped: | Cold Packs (Domestic, Overnight); Dry Ice (International) |
Table of Antisera Properties
| Antisera (antigen) | Affinity | Final use conc. | Detection Range (pg) |
| EAB25 (20E-BSA) | 20E | 1:40,000 | 20E: 30-2,000 |
| EAB27 (E-BSA) | E, 20E | 1:3,000 | E/20E: 4-500 |
Adapted from: McKinney DA, et al. (2017) Gen Comp Endocrinol. 253:60-69.
- McKinney DA, Strand MR, Brown MR. 2017 Evaluation of ecdysteroid antisera for a competitive enzyme immunoassay and extraction procedures for the measurement of mosquito ecdysteroids. Gen Comp Endocrinol. 253:60-69.
- Wang CF, Zhang Z, Sun W. Ecdysone oxidase and 3-dehydroecdysone-3β-reductase contribute to the synthesis of ecdysone during early embryonic development of the silkworm. Int J Biol Sci. 2018 Aug 6;14(11):1472-1482. View Article
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