Anti-E2F3 [LLF3#2G2] Antibody
This mouse monoclonal antibody was generated against human E2F3 (aa 1-244, 6X His-tagged) and recognizes both isoforms of human and mouse E2F3, E2F3a and E2F3b.
E2F3 is a winged helix transcription factor important for regulating the expression of genes encoding proteins required for cell cycle entry, DNA synthesis and mitosis and is an important target of pRB, a tumor suppressor protein. E2F3 can function in complexes that either repress or activate transcription. Studies using E2f3 mutant mice also show that E2F3 is important for cell cycle regulation, heart development and disease, cell migration and cancer. In addition in some human cancers E2F3 is over-expressed.
From a laboratory at Massachusetts Institute of Technology.
Part of The Investigator's Annexe program.
This mouse monoclonal antibody was generated against human E2F3 (aa 1-244, 6X His-tagged) and recognizes both isoforms of human and mouse E2F3, E2F3a and E2F3b.
E2F3 is a winged helix transcription factor important for regulating the expression of genes encoding proteins required for cell cycle entry, DNA synthesis and mitosis and is an important target of pRB, a tumor suppressor protein. E2F3 can function in complexes that either repress or activate transcription. Studies using E2f3 mutant mice also show that E2F3 is important for cell cycle regulation, heart development and disease, cell migration and cancer. In addition in some human cancers E2F3 is over-expressed.
From a laboratory at Massachusetts Institute of Technology.
Part of The Investigator's Annexe program.
| Product Type: | Antibody |
| Name: | E2F3 monoclonal antibody |
| Accession ID: | O00716 |
| Host: | Mouse |
| Isotype: | IgG |
| Clonality: | Monoclonal |
| Clone Name: | LLF3#2G2 |
| Specificity: | human, mouse |
| Immunogen: | Antibody was raised against 6x His-tagged human E2F3 (aa 1 - 244) |
| Format: | Liquid |
| Purification Method: | Protein G purified |
| Tested Applications: | ELISA, Western Blot, ChIP |
| Concentration: | 1mg/mL |
| Amount: | 100uL |
| Storage: | +4C or -20C (avoid repeated freeze / thaw cycles) |
| Shipped: | Cold packs |
Western Blot
Protein extracts were derived from mouse embryo fibroblasts (MEF)s in wild-type and E2f3a and E2f3b knockout littermates and probed with E2f3 monoclonal antibody (from LLF3#2G2).
- Danielian PS, Friesenhahn LB, Faust AM, West JC, Caron AM, Bronson RT, Lees, JA (2008) E2f3a and E2f3b make overlapping but different contributions to total E2f3 activity. Oncogene. 27(51): 6561 - 70
- Parisi T, Yuan TL, Faust AM, Caron AM, Bronson R, Lees JA (2007) Selective requirements for E2f3 in the development and tumorigenecity of Rb-deficient chimeric tissues. Mol. Cell. Bio. 27(6):2283 - 93
- Moberg K, Starz M, Lees JA (1996) E2F-4 switches from p130 to p107 and pRB in response to cell cycle reentry. Mol. Cell. Bio. 16(4): 1436 - 49
If you publish research with this product, please let us know so we can cite your paper.
