Cholesterol 22-hydroxylase (CYP90B27)
CYP90B27 is a cytochrome P450 enzyme that catalyzes the hydroxylation of cholesterol at position C-22 to form 22-hydroxycholesterol. CYP90B27 can also hydroxylate 27(25R)-hydroxycholesterol and 7?-hydroxycholesterol, also at position C-22.
From the laboratory of Toni M. Kutchan, PhD, and Megan Augustin, Donald Danforth Plant Science Center.
CYP90B27 is a cytochrome P450 enzyme that catalyzes the hydroxylation of cholesterol at position C-22 to form 22-hydroxycholesterol. CYP90B27 can also hydroxylate 27(25R)-hydroxycholesterol and 7?-hydroxycholesterol, also at position C-22.
From the laboratory of Toni M. Kutchan, PhD, and Megan Augustin, Donald Danforth Plant Science Center.
Specifications
| Product Type: | Protein |
| Name: | 22-Hydroxycholesterol 26-hydroxylase/oxidase |
| Accession ID: | KJ869252 |
| Source: | Sf9 Cells |
| Molecular Weight: | 54,919 |
| Buffer: | 100 mM tricine pH 7.5/ 5 mM thioglycolic acid |
| Comments: | Requires NADPH |
| Storage: | -80C |
| Shipped: | Dry Ice |
Provider
From the laboratory of Toni M. Kutchan, PhD, and Megan Augustin, Donald Danforth Plant Science Center.
References
- Augustin, M. M., D. R. Ruzicka, A. K. Shukla, J. M. Augustin, C. M. Starks, M. O'Neil-Johnson, M. R. McKain, B. S. Evans, M. D. Barrett, A. Smithson, G. K. Wong, M. K. Deyholos, P. P. Edger, J. C. Pires, J. H. Leebens-Mack, D. A. Mann and T. M. Kutchan (2015). "Elucidating steroid alkaloid biosynthesis in Veratrum californicum: production of verazine in Sf9 cells." Plant J 82(6): 991-1003.
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