Prostate Cancer Cell Line (22Rv1-undup2)
Human prostate cancer cell line, 22Rv1-undup2 is one of the cell clones isolated from 22Rv1 that is genetically corrected using TAL effector nuclease to delete the tandem duplication of 2b-exon3-CE1-3 of androgen receptor genome in 22Rv1 cell that drives the expression of androgen receptor variant 7 (ARv7).
Highlights:
- Expresses ARv7
- ARvs level is significantly reduced by this AR genomic correction
Prostate cancer is the development of cancer in the prostate, a gland in the male reproductive system. The androgen receptor (AR) helps prostate cancer cells to survive and is a target for many anti cancer research studies by looking at inhibition.
From the laboratory of Scott M. Dehm, PhD, University of Minnestora, Twin Cities.
Human prostate cancer cell line, 22Rv1-undup2 is one of the cell clones isolated from 22Rv1 that is genetically corrected using TAL effector nuclease to delete the tandem duplication of 2b-exon3-CE1-3 of androgen receptor genome in 22Rv1 cell that drives the expression of androgen receptor variant 7 (ARv7).
Highlights:
- Expresses ARv7
- ARvs level is significantly reduced by this AR genomic correction
Prostate cancer is the development of cancer in the prostate, a gland in the male reproductive system. The androgen receptor (AR) helps prostate cancer cells to survive and is a target for many anti cancer research studies by looking at inhibition.
From the laboratory of Scott M. Dehm, PhD, University of Minnestora, Twin Cities.
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| Product Type: | Cell Line |
| Name: | 22Rv1-undup2 |
| Cell Type: | Prostate Cancer |
| Morphology: | Epithelial cells |
| Organism: | Human |
| Source: | Prostate |
| Biosafety Level: | BSL 2 |
| Growth Conditions: | RPMI Medium 1640 - L-Glutamine (Invitrogen 11875), 10% FBS, 1xPen/Strep |
| Subculturing: | Seed 22Rv1-undup2 cell at 1X106/T75 flask, change medium twice a week and split 1 to 4 splitting once to twice a week. |
| Cryopreservation: | RPMI1640 , 20 % FBS and 10 %DMSO |
| Storage: | Liquid nitrogen |
| Shipped: | Dry Ice |
- Li Y, Alsagabi M, Fan D, Bova GS, Tewfik AH, Dehm SM. Intragenic rearrangement and altered RNA splicing of the androgen receptor in a cell-based model of prostate cancer progression. Cancer Res. 2011;71(6):21082117. doi:10.1158/0008-5472.CAN-10-1998.
- Van Etten JL, Nyquist M, Li Y, et al. Targeting a Single Alternative Polyadenylation Site Coordinately Blocks Expression of Androgen Receptor mRNA Splice Variants in Prostate Cancer. Cancer Res. 2017;77(19):52285235. doi:10.1158/0008-5472.CAN-17-0320.
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