RSK2 Knock Down RAW 264.7 Cell Line
Mouse RAW 264.7 cell line where the Ribosomal S6 Kinase 2 (RSK2) gene has been knocked down using shRNA.
Ribosomal S6 Kinase 2 (RSK2) is a member of the p90RSK family of serine/threonine kinases, which respond to many growth factors, peptide hormones, and neurotransmitters. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been involved in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry Syndrome (CLS), an X-linked mental retardation disorder.
From the laboratory of Zigang Dong, MD, DrPH, University Minnesota, Twin Cities.
Mouse RAW 264.7 cell line where the Ribosomal S6 Kinase 2 (RSK2) gene has been knocked down using shRNA.
Ribosomal S6 Kinase 2 (RSK2) is a member of the p90RSK family of serine/threonine kinases, which respond to many growth factors, peptide hormones, and neurotransmitters. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been involved in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry Syndrome (CLS), an X-linked mental retardation disorder.
From the laboratory of Zigang Dong, MD, DrPH, University Minnesota, Twin Cities.
This product is for sale to Nonprofit customers only. For profit customers, please Contact Us for more information.
| Product Type: | Cell Line |
| Name: | RSK2 Knockdown RAW264.7 Cells |
| Cell Type: | Macrophage; Abelson murine leukemia virus transformed |
| Accession ID: | P51812 |
| Morphology: | Monocyte/macrophage (adherent) |
| Organism: | Mouse |
| Source: | Abelson murine leukemia virus-induced tumor; ascites |
| Biosafety Level: | BSL 2 |
| Growth Conditions: | Dulbecco's Modified Eagle's Medium with 10% fetal bovine serum |
| Subculturing: | Subcultures are prepared by scraping. For a 75 cm2 flask, remove all but 10 mL culture medium (adjust amount accordingly for other culture vessels). Dislodge cells from the flask substrate with a cell scraper; aspirate and add appropriate aliquots of the cell suspension into new culture vessels. |
| Cryopreservation: | Complete growth medium supplemented with 5% (v/v) DMSO |
| Age: | Adult male |
| Comments: | This cell line is a suitable transfection host. The cells will pinocytose neutral red and will phagocytose latex beads and zymosan. They are capable of antibody dependent lysis of sheep erythrocytes and tumor cell targets. LPS or PPD treatment for 2 days stimulates lysis of erythrocytes but not tumor cell targets. |
| Storage: | Liquid Nitrogen |
| Shipped: | Dry Ice |
- Yao K, Lee SY, Peng C, Lim DY, Yamamoto H, Ryu J, Lim TG, Chen H, Jin G, Zhao Z, Han Y, Ma WY, Bode AM, Dong Z. RSK2 is required for TRAF6 phosphorylation-mediated colon inflammation. Oncogene. 2018 Jun;37(26):3501-3513. doi: 10.1038/s41388-018-0167-6. Epub 2018 Mar 22. PubMed PMID: 29563609; PubMed Central PMCID: PMC6023753./li>
If you publish research with this product, please let us know so we can cite your paper.
