PKR KO (C57) Macrophage Cell Line
A protein kinase R (PKR) KO macrophage cell line isolated from bone marrow in C57BL/6 mice after immortalization with a retrovirus (J2) containing the myc and v-raf oncogenes.
Protein kinase-R is activated by double-stranded RNA (dsRNA), introduced to the cells by a viral infection. PKR contains an N-terminal dsRNA binding domain (dsRBD) and a C-terminal kinase domain, that gives it pro-apoptotic (cell-killing) functions. C57BL/6 also known as C57 black 6, C57 or black 6, is a common inbred strain of laboratory mouse. They are the most widely used and best-selling mouse strain, due to the availability of congenic strains, easy breeding, and robustness. 129 mice are widely used in the production of targeted mutations due to the availability of multiple embryonic stem cell lines derived from them. They also have a high incidence of spontaneous testicular teratomas.
From the laboratory of Howard A. Young, PhD, National Cancer Institute/NIH.
A protein kinase R (PKR) KO macrophage cell line isolated from bone marrow in C57BL/6 mice after immortalization with a retrovirus (J2) containing the myc and v-raf oncogenes.
Protein kinase-R is activated by double-stranded RNA (dsRNA), introduced to the cells by a viral infection. PKR contains an N-terminal dsRNA binding domain (dsRBD) and a C-terminal kinase domain, that gives it pro-apoptotic (cell-killing) functions. C57BL/6 also known as C57 black 6, C57 or black 6, is a common inbred strain of laboratory mouse. They are the most widely used and best-selling mouse strain, due to the availability of congenic strains, easy breeding, and robustness. 129 mice are widely used in the production of targeted mutations due to the availability of multiple embryonic stem cell lines derived from them. They also have a high incidence of spontaneous testicular teratomas.
From the laboratory of Howard A. Young, PhD, National Cancer Institute/NIH.
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| Product Type: | Cell Line |
| Name: | PKR KO (C57) Macrophage Cell Line |
| Cell Type: | Macrophage |
| Accession ID: | P19525 |
| Morphology: | Adherent |
| Organism: | Mouse |
| Source: | Bone Marrow |
| Biosafety Level: | BSL2 |
| Growth Conditions: | DMEM + 10% FBS + Glutamine |
| Cryopreservation: | 90% FBS + 10% DMSO |
| Storage: | Liquid Nitrogen |
| Shipped: | Dry Ice |
- Blasi E, Radzioch D, Merletti L, Varesio L. Generation of macrophage cell line from fresh bone marrow cells with a myc/raf recombinant retrovirus. Cancer Biochem Biophys. 1989;10(4):303-317.
- Blasi E, Mathieson BJ, Varesio L, Cleveland JL, Borchert PA, Rapp UR. Selective immortalization of murine macrophages from fresh bone marrow by a raf/myc recombinant murine retrovirus. Nature. 1985;318(6047):667-670.
- Xiao C, Wang RH, Lahusen TJ, et al. Progression of chronic liver inflammation and fibrosis driven by activation of c-JUN signaling in Sirt6 mutant mice. J Biol Chem. 2012;287(50):41903-41913.
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