STAT1 KO (129/SvEV) Macrophage Cell Line
A knockout signal transducer and activator of transcription 1 (STAT1) macrophage cell line isolated from bone marrow in 129/SvEV mice after immortalization with a retrovirus (J2) containing the myc and v-raf oncogenes.
All STAT molecules are phosphorylated by receptor associated kinases, that causes activation, dimerization by forming homo- or heterodimers and finally translocate to nucleus to work as transcription factors. Specifically STAT1 can be activated by several ligands such as Interferon alpha (IFNα), Interferon gamma (IFNγ), Epidermal Growth Factor (EGF), Platelet Derived Growth Factor (PDGF) or Interleukin 6 (IL-6). 129 mice are widely used in the production of targeted mutations due to the availability of multiple embryonic stem cell lines derived from them. They also have a high incidence of spontaneous testicular teratomas.
From the laboratory of Howard A. Young, PhD, National Cancer Institute/NIH.
A knockout signal transducer and activator of transcription 1 (STAT1) macrophage cell line isolated from bone marrow in 129/SvEV mice after immortalization with a retrovirus (J2) containing the myc and v-raf oncogenes.
All STAT molecules are phosphorylated by receptor associated kinases, that causes activation, dimerization by forming homo- or heterodimers and finally translocate to nucleus to work as transcription factors. Specifically STAT1 can be activated by several ligands such as Interferon alpha (IFNα), Interferon gamma (IFNγ), Epidermal Growth Factor (EGF), Platelet Derived Growth Factor (PDGF) or Interleukin 6 (IL-6). 129 mice are widely used in the production of targeted mutations due to the availability of multiple embryonic stem cell lines derived from them. They also have a high incidence of spontaneous testicular teratomas.
From the laboratory of Howard A. Young, PhD, National Cancer Institute/NIH.
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| Product Type: | Cell Line |
| Name: | STAT1 KO (129/SvEV) |
| Cell Type: | Macrophage |
| Accession ID: | P42224 |
| Morphology: | Adherent |
| Organism: | Mouse |
| Source: | Bone Marrow |
| Biosafety Level: | BSL2 |
| Growth Conditions: | DMEM + 10% FBS + Glutamine |
| Cryopreservation: | 90% FBS + 10% DMSO |
| Storage: | Liquid Nitrogen |
| Shipped: | Dry Ice |
- Blasi E, Radzioch D, Merletti L, Varesio L. Generation of macrophage cell line from fresh bone marrow cells with a myc/raf recombinant retrovirus. Cancer Biochem Biophys. 1989;10(4):303-317.
- Blasi E, Mathieson BJ, Varesio L, Cleveland JL, Borchert PA, Rapp UR. Selective immortalization of murine macrophages from fresh bone marrow by a raf/myc recombinant murine retrovirus. Nature. 1985;318(6047):667-670.
- Xiao C, Wang RH, Lahusen TJ, et al. Progression of chronic liver inflammation and fibrosis driven by activation of c-JUN signaling in Sirt6 mutant mice. J Biol Chem. 2012;287(50):41903-41913.
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