Ad CMV Cre
Ad Cre expresses Cre under control of a CMV promoter.
Ready-made control adenoviruses (Ad) are generally in stock and can ship anytime. These particles are CsCl purified and supplied in 100 uL aliquots at 1X10^12 pts/mL. These preps are RCA free and PFU titered.
Ad Cre expresses Cre under control of a CMV promoter.
Ready-made control adenoviruses (Ad) are generally in stock and can ship anytime. These particles are CsCl purified and supplied in 100 uL aliquots at 1X10^12 pts/mL. These preps are RCA free and PFU titered.
Specifications
| Product Type: | Virus |
| Name: | Ad CMV Cre |
| Biosafety Level: | BSL-2 |
| Vector Information: | Recombinant adenovirus |
| Target Gene / Reporter(s): | Cre |
| Promoter: | CMV |
| Virus: | Adenovirus |
| Titer: | 1X10^12 pts/mL, CsCl purified, RCA free and PFU titred |
| Storage: | Store at -80C. Avoid multiple freeze/thaw cycles.Virus is supplied at 1X10^12 particles/mL. We recommend thawing the virus at room temperature. For aliquoting, 0.5 mL screw cap vials are recommended. Other tubes do not seal tight enough and eventually the solution desiccates at -80 and the virus looses infectivity. We do not recommend diluting the virus and refreezing unless you use the cryo-protective buffer in the original formulation. Do not store or thaw the virus on ice, -20 C or 4 C. |
| Shipped: | Frozen on Dry ice |
Comments
Ad CMV Cre #17187Cells should be infected when ~ 80% confluent and the normal growth media replaced with media containing only 2% serum. Media can be supplemented or replaced with normal growth media after 4 hrs if required. Wait at least 48 hrs prior to analysis by IHC, WB, ELISA etc.
References
- Lua I, Li Y, Pappoe LS, Asahina K. Myofibroblastic Conversion and Regeneration of Mesothelial Cells in Peritoneal and Liver Fibrosis. Am J Pathol. 2015 Dec;185(12):3258-73. View Article
- Li Y, Lua I, French SW, Asahina K. Role of TGF-β signaling in differentiation of mesothelial cells to vitamin A-poor hepatic stellate cells in liver fibrosis. Am J Physiol Gastrointest Liver Physiol. 2016 Feb 15;310(4):G262-72. View Article
- Anderson RD, Haskell RE, Xia H, Roessler BJ, and Davidson BL. A Simple Method for the Rapid Generation of Recombinant Adenovirus Vectors. Gene Therapy. (2000) 7, 1034-1038.
- Hennigan RF, Fletcher JS, Guard S, Ratner N. Proximity biotinylation identifies a set of conformation-specific interactions between Merlin and cell junction proteins. Sci Signal. 2019 Apr 23;12(578). pii: eaau8749. View Article
- Zingg B, Peng B, Huang J, Tao HW, Zhang LI. Synaptic Specificity and Application of Anterograde Transsynaptic AAV for Probing Neural Circuitry. J Neurosci. 2020 Apr 15;40(16):3250-3267. View article
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