Ad CMV Cre - RSV eGFP

Ad CMV IL-24

Ad CMV Cre - RSV eGFP expresses Cre via a CMV promoter in the E1 region of the virus and expresses eGFP from an RSV promoter in the E3 region.

Ready-made control adenoviruses (Ad) are generally in stock and can ship anytime. These particles are CsCl purified and supplied in 100 uL aliquots at 1X10^12 pts/mL. These preps are RCA free and PFU titered.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
FVQ005
Ad CMV Cre - RSV eGFP
100uL (1x10^12 PFU/mL) In stock
Regular Price:$500.00
On Sale:
Specifications

Product Type: Virus
Name: Ad CMV Cre - RSV eGFP
Biosafety Level: BSL-2
Vector Information: Recombinant adenovirus
Target Gene / Reporter(s): Cre, eGFP
Promoter: CMV (Cre), RSV (eGFP)
Virus: Adenovirus
Titer: 1X10^12 pts/mL, CsCl purified, RCA free and PFU titred
Storage: Store at -80C. Avoid multiple freeze/thaw cycles.Virus is supplied at 1X10^12 particles/mL. We recommend thawing the virus at room temperature. For aliquoting, 0.5 mL screw cap vials are recommended. Other tubes do not seal tight enough and eventually the solution desiccates at -80 and the virus looses infectivity. Aliquots of less than 50uL are unstable and should be avoided. We do not recommend diluting the virus and refreezing unless you use the cryo-protective buffer in the original formulation. Do not store or thaw the virus on ice, -20 C or 4 C.
Shipped: Frozen on Dry ice

Comments

Ad CMV Cre RSV GFP#17186

Cells should be infected when ~ 80% confluent and the normal growth media replaced with media containing only 2% serum. Media can be supplemented or replaced with normal growth media after 4 hrs if required. Wait at least 48 hrs prior to analysis by IHC, WB, ELISA etc.

References
  1. Anderson RD, Haskell RE, Xia H, Roessler BJ, and Davidson BL. A Simple Method for the Rapid Generation of Recombinant Adenovirus Vectors. Gene Therapy. (2000) 7, 1034-1038.
  2. Valapala M, Hose S, Gongora C, Dong L, Wawrousek EF, Samuel Zigler J Jr, Sinha D. Impaired endolysosomal function disrupts Notch signalling in optic nerve astrocytes. Nat Commun. 2013;4:1629. View Article
  3. Su T, Bondar T, Zhou X, Zhang C, He H, Medzhitov R. Two-signal requirement for growth-promoting function of Yap in hepatocytes. Elife. 2015 Feb 10;4. View Article
  4. Cai J, Pires KM, Ferhat M, Chaurasia B, Buffolo MA, Smalling R, Sargsyan A, Atkinson DL, Summers SA, Graham TE, Boudina S. Autophagy Ablation in Adipocytes Induces Insulin Resistance and Reveals Roles for Lipid Peroxide and Nrf2 Signaling in Adipose-Liver Crosstalk. Cell Rep. 2018 Nov 13;25(7):1708-1717.e5. View Article

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