Xenopus Carcass Fibroblast Cell Line (XTC-2[pEF-BirAV5])
![Xenopus Carcass Fibroblast Cell Line (XTC-2[pEF-BirAV5])](https://www.kerafast.com/MediaStorage/Product/Images/Large/3570_2001202001281435690.jpg "Xenopus Carcass Fibroblast Cell Line (XTC-2[pEF-BirAV5])")
XTC-2[pEF-BirAV5] is an adult Xenopus laevis carcass fibroblast cell line; engineered to express the biotin ligase BirAV5.
Highlights:
- Can be used to express biotinylated proteins for chromatin precipitation sequencing, protein-protein interactions, etc.
From the laboratory of Robert J. Denver, PhD, University of Michigan.
XTC-2[pEF-BirAV5] is an adult Xenopus laevis carcass fibroblast cell line; engineered to express the biotin ligase BirAV5.
Highlights:
- Can be used to express biotinylated proteins for chromatin precipitation sequencing, protein-protein interactions, etc.
From the laboratory of Robert J. Denver, PhD, University of Michigan.
Specifications
| Product Type: | Cell Line |
| Name: | XTC-2[pEF-BirAV5] |
| Cell Type: | Fibroblast |
| Organism: | Xenopus laevis |
| Source: | Carcass |
| Biosafety Level: | BSL1 |
| Subculturing: | Can be passaged with trypsin per standard protocols |
| Growth Conditions: |
Experiment dependent: Leibovitz L-15 medium (0.66X amphibian strength, diluted 1:1.5) supplemented with sodium bicarbonate (2.2 g/L), penicillin G (100 U/mL), streptomycin sulfate (100 μg/mL), and 10% fetal bovine serum; 5%CO2, 25C. Also see: Bagamasbad PD, Bonett RM, Sachs L, et al. Mol Endocrinol. 2015 Jun;29(6):856-72. Or, Leibovitz (L15) medium (0.66X amphibian strength) with 10% thyroid hormone stripped FBS; 5% CO2, 25C. See COMMENTS section. |
| Cryopreservation: | L-15 growth medium with 10% DMSO |
| Comments: | This cell line can be used to express biotinylated proteins for chromatin precipitation sequencing, protein-protein interactions, etc. Transfect the cells with vectors that express fusion proteins containing the BIO peptide for biotinylation. |
| Storage: | LN2 |
| Shipped: | Dry Ice |
Provider
From the laboratory of Robert J. Denver, PhD, University of Michigan.
Comments
Notes from the provider: L-15 works well, but the cells can be cultured in other media like DMEM and MEM. These cells can be cultured in unstripped FBS. The decision to strip thyroid hormones (using resin) or steroids (using charcoal) depends on what experiment one is doing (i.e., adding back the hormone to look at physiological or transcriptional responses).
References
If you publish research with this product, please let us know so we can cite your paper.
