Mouse Neuronal Cell Line (HT-22 FLBIO-rKLF9/SV40-BirAV5)
HT22-FLBIO-rKLF9/BirAV5 is an adult mouse hippocampus-derived neuronal cell line; engineered to express the biotin ligase BirAV5 and rat Kruppel-like factor 9 (KLF9; becomes biotinylated in vivo).
From the laboratory of Robert J. Denver, PhD, University of Michigan.
HT22-FLBIO-rKLF9/BirAV5 is an adult mouse hippocampus-derived neuronal cell line; engineered to express the biotin ligase BirAV5 and rat Kruppel-like factor 9 (KLF9; becomes biotinylated in vivo).
From the laboratory of Robert J. Denver, PhD, University of Michigan.
Specifications
| Product Type: | Cell Line |
| Name: | HT22-FLBIO-rKLF9/SV40-BirAV5 |
| Cell Type: | Neuron |
| Accession ID: | Q13886 |
| Organism: | Mouse |
| Source: | Adult hippocampus; pSV40 immortalized |
| Biosafety Level: | BSL1 |
| Subculturing: | Can be passaged with trypsin per standard protocols |
| Growth Conditions: | High-glucose DMEM (Invitrogen) supplemented with 10% fetal bovine serum, penicillin G (100 U/ml) and streptomycin sulfate (100 ug/ml) under a humidified atmosphere of 5% CO2 at 37C. Maintain selection with 100 ug/ml zeocin plus 1 ug/ml puromycin. Please note that HT-22 cells are resistant to G-418. |
| Cryopreservation: | DMEM growth medium with 10% DMSO |
| Comments: | This cell line expresses biotinylated KLF9 for chromatin precipitation, protein-protein interactions, etc. |
| Storage: | LN2 |
| Shipped: | Dry Ice |
Provider
From the laboratory of Robert J. Denver, PhD, University of Michigan.
References
- Knoedler, J.R., Subramani, A. and Denver, R.J. (2017) The Kruppel-like factor 9 cistrome in mouse hippocampal neurons reveals predominant transcriptional repression via proximal promoter binding. BMC Genomics 18:299. doi: 10.1186/s12864-017-3640-7 PMID:28407733.
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