Mouse Neuronal Cell Line (HT-22 [TR/To-mKlf13])
![Mouse Neuronal Cell Line (HT-22 [TR/To-mKlf13])](https://www.kerafast.com/MediaStorage/Product/Images/Large/3570_2001202001281435690.jpg "Mouse Neuronal Cell Line (HT-22 [TR/To-mKlf13])")
HT-22 [TR/TO-mKlf13] is an adult mouse hippocampus-derived neuronal cell line; engineered to express rat/mouse Krüppel-like factor 9 under the control of the tet repressor (the gene is induced by doxycycline).
From the laboratory of Robert J. Denver, PhD, University of Michigan.
HT-22 [TR/TO-mKlf13] is an adult mouse hippocampus-derived neuronal cell line; engineered to express rat/mouse Krüppel-like factor 9 under the control of the tet repressor (the gene is induced by doxycycline).
From the laboratory of Robert J. Denver, PhD, University of Michigan.
Specifications
| Product Type: | Cell Line |
| Name: | HT-22 [TR/To-mKlf13] |
| Cell Type: | Neuron |
| Accession ID: | Q9Y2Y9 |
| Organism: | Mouse |
| Source: | Adult hippocampus; pSV40 immortalized |
| Biosafety Level: | BSL1 |
| Subculturing: | Can be passaged with trypsin per standard protocols |
| Growth Conditions: | High-glucose DMEM (Invitrogen) supplemented with 10% fetal bovine serum, penicillin G (100 U/ml) and streptomycin sulfate (100 ug/ml) under a humidified atmosphere of 5% CO2 at 37C. Maintain selection with 100 ug/ml zeocin plus 5 ug/ml blasticidin. Please note that HT-22 cells are resistant to G-418. |
| Cryopreservation: | DMEM growth medium with 10% DMSO |
| Comments: | This cell line expresses KLF13 under the control of tetracycline (or doxycycline) for RNA sequencing, etc. |
| Storage: | LN2 |
| Shipped: | Dry Ice |
Provider
From the laboratory of Robert J. Denver, PhD, University of Michigan.
References
- Avila-Mendoza, J., Subramani, A., Sifuentes, C.J. and Denver, R.J. (2020) Molecular mechanisms for Kruppel-like factor 13 actions in hippocampal neurons. Molecular Neurobiology doi: 10.1007/s12035-020-01971-w.
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