Mouse Neuronal Cell Line (HT-22 Klf9/Klf13 KO)
HT-22 Klf9/Klf13 double KO is an adult mouse hippocampus-derived neuronal cell line; CRISPR/Cas9 mutation of Klf9 to create a functional knockout.
From the laboratory of Robert J. Denver, PhD, University of Michigan.
HT-22 Klf9/Klf13 double KO is an adult mouse hippocampus-derived neuronal cell line; CRISPR/Cas9 mutation of Klf9 to create a functional knockout.
From the laboratory of Robert J. Denver, PhD, University of Michigan.
Specifications
| Product Type: | Cell Line |
| Name: | HT-22 Klf9/Klf13 KO |
| Cell Type: | Neuron |
| Accession ID: | Q9Y2Y9 |
| Organism: | Mouse |
| Source: | Adult hippocampus; pSV40 immortalized |
| Biosafety Level: | BSL1 |
| Subculturing: | Can be passaged with trypsin per standard protocols |
| Growth Conditions: | High-glucose DMEM (Invitrogen) supplemented with 10% fetal bovine serum, penicillin G (100 U/ml) and streptomycin sulfate (100 ug/ml) under a humidified atmosphere of 5% CO2 at 37C. Please note that HT-22 cells are resistant to G-418. |
| Cryopreservation: | DMEM growth medium with 10% DMSO |
| Storage: | LN2 |
| Shipped: | Dry Ice |
Provider
From the laboratory of Robert J. Denver, PhD, University of Michigan.
References
- Knoedler, J.R., Avila-Mendoza, J., Subramani, A., C. and Denver, R.J. (2020) The paralogous Krüppel-like factors 9 and 13 regulate the mammalian cellular circadian clock output gene Dbp. Journal of Biological Rhythms doi: 10.1177/0748730420913205 PMID:32241200 PMID:28407733.
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