Mouse Neuronal Cell Line (HT-22 BirAV5/3xBTE-Luc)
![Mouse Neuronal Cell Line (HT-22 [BirAV5/3xBTE-Luc])](https://www.kerafast.com/MediaStorage/Product/Images/Large/3570_2001202001281435690.jpg "Mouse Neuronal Cell Line (HT-22 BirAV5/3xBTE-Luc)")
HT22[BirAV5/3xBTE-Luc] Adult mouse hippocampus-derived neuronal cell line; stably expresses biotin ligase BirA, and stably transfected with luciferase reporter with 3 basic transcription element (BTE) repeats.
From the laboratory of Robert J. Denver, PhD, University of Michigan.
HT22[BirAV5/3xBTE-Luc] Adult mouse hippocampus-derived neuronal cell line; stably expresses biotin ligase BirA, and stably transfected with luciferase reporter with 3 basic transcription element (BTE) repeats.
From the laboratory of Robert J. Denver, PhD, University of Michigan.
Specifications
| Product Type: | Cell Line |
| Name: | HT-22 [BirAV5/3xBTE-Luc] |
| Cell Type: | Neuron |
| Morphology: | Neuronal |
| Organism: | Mouse |
| Source: | Adult hippocampus; pSV40 immortalized |
| Biosafety Level: | BSL1 |
| Subculturing: | Can be passaged with trypsin per standard protocols |
| Growth Conditions: | High-glucose DMEM (Invitrogen) supplemented with 10% fetal bovine serum, penicillin G (100 U/ml) and streptomycin sulfate (100 _g/ml) under a humidified atmosphere of 5% CO2 at 37C. Maintain selection with 100 _g/ml zeocin. Please note that HT-22 cells are resistant to G-418. |
| Cryopreservation: | DMEM growth medium with 10% DMSO |
| Comments: | The HT22-pSV40-BirAV5 cell line was stably transfected with pGL4.23-3xBTE; this cell line reports (via luciferase) activation or repression mediated by the basic transcription element to which KLF9 and other KLF/Sp factors bind. |
| Storage: | LN2 |
| Shipped: | Dry Ice |
Provider
From the laboratory of Robert J. Denver, PhD, University of Michigan.
References
- Knoedler, J.R., Subramani, A. and Denver, R.J. (2017) The Krppel-like factor 9 cistrome in mouse hippocampal neurons reveals predominant transcriptional repression via proximal promoter binding. BMC Genomics 18:299. doi: 10.1186/s12864-017-3640-7 PMID:28407733.
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