Mouse Neuroblastoma Cell Line (N2a[TRb1]-274 A+B Clone AB-2)
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Neuro2a[TRb1]-274 Mouse neuroblastoma cell lines stably expressing human thyroid hormone receptor beta 1 with mutations in the thyroid hormone response element (TRE) of the Dnmt3a gene introduced by CRISPR/Cas9 genome editing.
From the laboratory of Robert J. Denver, PhD, University of Michigan.
Neuro2a[TRb1]-274 Mouse neuroblastoma cell lines stably expressing human thyroid hormone receptor beta 1 with mutations in the thyroid hormone response element (TRE) of the Dnmt3a gene introduced by CRISPR/Cas9 genome editing.
From the laboratory of Robert J. Denver, PhD, University of Michigan.
Specifications
| Product Type: | Cell Line |
| Name: | N2a[TRb1-274A+B Clone AB-2] |
| Cell Type: | Neuron |
| Morphology: | Neuronal |
| Organism: | Mouse |
| Source: | Mouse neuroblastoma |
| Biosafety Level: | BSL1 |
| Growth Conditions: | DMEM/F12 supplemented with hygromycin B (500 ug/mL) and 10% fetal bovine serum stripped of thyroid hormone. |
| Subculturing: | Can be passaged with trypsin per standard protocols |
| Cryopreservation: | DMEM/F12 growth medium with 10% DMSO |
| Comments: | Mutation of the TRE in the Dnmt3a gene abolished the transcriptional response to thyroid hormone (T3). |
| Storage: | LN2 |
| Shipped: | Dry Ice |
Provider
From the laboratory of Robert J. Denver, PhD, University of Michigan.
References
- Kyono, Y., Subramani, A., Ramadoss, P., Hollenberg, A.N., Bonett, R.M. and Denver, R.J. (2016) Liganded thyroid hormone receptors transactivate the DNA methyltransferase 3a gene in mouse neuronal cells. Endocrinology 157:3647-3657 doi: 10.1210/en.2015-1529 PMID:27387481
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