Ad CMV eGFP - RSV Luc
Ad CMV eGFP - RSV Luc expresses eGFP via a CMV promoter in the E1 region of the virus and expresses luciferase from an RSV promoter in the E3 region.
Ready-made control adenoviruses (Ad) are generally in stock and can ship anytime. These particles are CsCl purified and supplied in 100 uL aliquots at 1X10^12 pts/mL. These preps are RCA free and PFU titered.
Ad CMV eGFP - RSV Luc expresses eGFP via a CMV promoter in the E1 region of the virus and expresses luciferase from an RSV promoter in the E3 region.
Ready-made control adenoviruses (Ad) are generally in stock and can ship anytime. These particles are CsCl purified and supplied in 100 uL aliquots at 1X10^12 pts/mL. These preps are RCA free and PFU titered.
Specifications
| Product Type: | Virus |
| Name: | Ad CMV eGFP - RSV Luc |
| Biosafety Level: | BSL-2 |
| Vector Information: | Recombinant adenovirus |
| Target Gene / Reporter(s): | eGFP, Luc |
| Promoter: | CMV (eGFP); RSV (Luc) |
| Virus: | Adenovirus |
| Titer: | 1X10^12 pts/mL, CsCl purified, RCA free and PFU titred |
| Storage: | Store at -80C. Avoid multiple freeze/thaw cycles.Virus is supplied at 1X10^12 particles/mL. We recommend thawing the virus at room temperature. For aliquoting, 0.5 mL screw cap vials are recommended. Other tubes do not seal tight enough and eventually the solution desiccates at -80 and the virus looses infectivity. We do not recommend diluting the virus and refreezing unless you use the cryo-protective buffer in the original formulation. Do not store or thaw the virus on ice, -20 C or 4 C. |
| Shipped: | Frozen on Dry ice |
Comments
Cells should be infected when ~ 80% confluent and the normal growth media replaced with media containing only 2% serum. Media can be supplemented or replaced with normal growth media after 4 hrs if required. Wait at least 48 hrs prior to analysis by IHC, WB, ELISA etc.
References
- Anderson RD, Haskell RE, Xia H, Roessler BJ, and Davidson BL. A Simple Method for the Rapid Generation of Recombinant Adenovirus Vectors. Gene Therapy. (2000) 7, 1034-1038.
- Cheung J, Chino T, Co C, Konopka K, Düzgünes N. A nonviral vector with transfection activity comparable with adenoviral transduction. Ther Deliv. 2016 Nov;7(11):739-749. PubMed PMID: 27790941. View Article
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