Translation and Tethering (TnT) Plasmid
The TnT plasmid is a nucleic acid-based biosensor to test the specific impacts of translation control factors by microscopy.
Highlights:
- Combination of the nascent chain tracking translation reporter and mRNA tethering reporters
- Adaptable to any factor since tethering/recruitment is through a tethering cassette
- Useful for screening translation as well as up/down regulation of proteins
- Study translation factors as well as effects of subcellular localization
The "Translation and Tethering" TnT biosensor is a combination of the nascent chain tracking translation reporter and mRNA tethering reporters. The TnT biosensor is adaptable to any factor since tethering/recruitment is through a tethering cassette and not the endogenous RNA binding domain of a specific factor. TnT can be used to screen effects such as translation as well as up/down regulation of proteins. Additionally, TnT can be used to study translation factors as well as effects of subcellular localization on translation by tethering TnT mRNA to a specific location.
From the laboratory of Timothy J. Stasevich, PhD, Colorado State University.
The TnT plasmid is a nucleic acid-based biosensor to test the specific impacts of translation control factors by microscopy.
Highlights:
- Combination of the nascent chain tracking translation reporter and mRNA tethering reporters
- Adaptable to any factor since tethering/recruitment is through a tethering cassette
- Useful for screening translation as well as up/down regulation of proteins
- Study translation factors as well as effects of subcellular localization
The "Translation and Tethering" TnT biosensor is a combination of the nascent chain tracking translation reporter and mRNA tethering reporters. The TnT biosensor is adaptable to any factor since tethering/recruitment is through a tethering cassette and not the endogenous RNA binding domain of a specific factor. TnT can be used to screen effects such as translation as well as up/down regulation of proteins. Additionally, TnT can be used to study translation factors as well as effects of subcellular localization on translation by tethering TnT mRNA to a specific location.
From the laboratory of Timothy J. Stasevich, PhD, Colorado State University.
| Product Type: | Plasmid |
| Name: | pUb_smF-KDM_15xBoxB_24xms2 TnT Plasmid |
| Gene/insert name: | Spaghetti-monster-10xFLAG-hKDM5B |
| Organism: | KDM5B-human |
| Antibiotic Resistance: | Amp |
| Fusion Tag(s): | 10xFLAG |
| Vector Information: | mRNA: promoter: human Ubiquitin, ORF: spaghetti-monster-10xFLAG-hKDM5B, Actin 3'UTR, 15xBoxB stem loops inserted in the Actin UTR at AgeI cut site, 24xMS2 after (3' of) the UTR, WPRE posttranslational regulatory element |
| Grow in E. coli at 37 C: | No. Grow at 30C in NEB standard |
| Selectable markers: | Amp |
| Cloning Site 5': | AsiSI |
| Cloning Site 3': | NheI or PmeI |
| Insert Size: | 5655bp |
| Vector Backbone and Size: | 13,920bp |
| High or low copy: | Low |
| Storage: | -20C |
| Shipped: | Room temperature |
- Charlotte A. Cialek, Tatsuya Morisaki, Ning Zhao, Taiowa A. Montgomery, Timothy J. Stasevich. Imaging translational control by Argonaute with single-molecule resolution in live cells. bioRxiv 2021.04.30.442135; doi: https://doi.org/10.1101/2021.04.30.442135
If you publish research with this product, please let us know so we can cite your paper.
