pBS-L-ФT Plasmid
The plasmid pBS-L-ΦT encodes the large subunit (L) of the vesicular stomatitis virus (VSV) polymerase protein expressed from the bacteriophage T7 promoter in pBS, which has been further modified to contain a T7 terminator sequence cloned between the SacII and SacI restriction sites in pBS-SK+ [1]. The plasmid is used as part of the VSV reverse genetics system to recover recombinant VSV from plasmids [2, 3]. The recovery of rVSV involves the transfection of vaccinia-T7 infected cells with 4 (or 5) different plasmids; one that expresses the rVSV antigenome (e.g. pVSV-FL+(2) or pVSV-ΔG-GFP) and 3 (or 4) other plasmids that express the VSV N, P, and L proteins (or N, P, L and G for replication-restrictedΔG-VSV).
From the laboratory of Michael A. Whitt, Ph.D., University of Tennessee.
Part of The Investigator's Annexe program.
The plasmid pBS-L-ΦT encodes the large subunit (L) of the vesicular stomatitis virus (VSV) polymerase protein expressed from the bacteriophage T7 promoter in pBS, which has been further modified to contain a T7 terminator sequence cloned between the SacII and SacI restriction sites in pBS-SK+ [1]. The plasmid is used as part of the VSV reverse genetics system to recover recombinant VSV from plasmids [2, 3]. The recovery of rVSV involves the transfection of vaccinia-T7 infected cells with 4 (or 5) different plasmids; one that expresses the rVSV antigenome (e.g. pVSV-FL+(2) or pVSV-ΔG-GFP) and 3 (or 4) other plasmids that express the VSV N, P, and L proteins (or N, P, L and G for replication-restrictedΔG-VSV).
From the laboratory of Michael A. Whitt, Ph.D., University of Tennessee.
Part of The Investigator's Annexe program.
| Product Type: | Plasmid |
| Gene/insert name: | VSV large polymerase subunit (L) |
| Accession ID: | Q8B0H0 |
| Antibiotic Resistance: | Ampicillin or Kanamycin (please see vial label and packing slip) |
| Fusion Tag(s): | none |
| Concentration: | 100uL (100ng/uL) |
| Grow in E. coli at 37 C: | Yes |
| Cloning Site 5': | XhoI |
| Cloning Site 3': | XbaI |
| Insert Size: | 6,446 bp |
| Vector Backbone and Size: | pBS-SK-ΦT, 3105 bp |
| High or low copy: | High |
| Comments: | For suggested protocol, see: Whitt, MA, J. Virol. Methods, 2010. 169(2): p. 365-74. |
| Shipped: | Ambient temperature |
- Stillman, E.A., J.K. Rose, and M.A. Whitt, Replication and amplification of novel vesicular stomatitis virus minigenomes encoding viral structural proteins. J. Virol., 1995. 69: p. 2946-2953.
- Lawson, N.D., et al., Recombinant vesicular stomatitis viruses from DNA. Proc.Natl.Acad.Sci.(USA), 1995. 92(10): p. 4477-4481.
- Whitt, M.A., Generation of VSV pseudotypes using recombinant DeltaG-VSV for studies on virus entry, identification of entry inhibitors, and immune responses to vaccines. J. Virol. Methods, 2010. 169(2): p. 365-74.
- Lee SS, Phy K, Peden K, Sheng-Fowler L. Development of a micro-neutralization assay for ebolaviruses using a replication-competent vesicular stomatitis hybrid virus and a quantitative PCR readout. Vaccine. 2017 Apr 17. pii: S0264-410X(17)30325-0. View Article
- Kane M, Deiss F, Chervonsky A, Golovkina TV. A Single Locus Controls Interferon Gamma-Independent Antiretroviral Neutralizing Antibody Responses. J Virol. 2018 Jul 31;92(16). pii: e00725-18. doi: 10.1128/JVI.00725-18. Print 2018 Aug 15. View Article
- Rihn SJ, Aziz MA, Stewart DG, et al. TRIM69 Inhibits Vesicular Stomatitis Indiana Virus. J Virol. 2019;93(20):e00951-19. Published 2019 Sep 30. View article
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