pBS-G-ФT Plasmid
The plasmid pBS-G-ΦT encodes the vesicular stomatitis virus (VSV) glycoprotein (G) from the Indiana serotype expressed from the bacteriophage T7 promoter in pBS, which has been further modified to contain a T7 terminator sequence cloned between the SacII and SacI restriction sites in pBS-SK+ [1]. The plasmid is used as part of the VSV reverse genetics system to recover replication-restricted ΔG-VSV from plasmids [2]. The recovery of ΔG-VSV involves the transfection of cells that have been infected with recombinant vaccinia expressing bacteriophage T7 RNA polymerase (vvT7) with 5 different plasmids; one which expresses the ΔG-VSV antigenomic-sense (or positive-sense) RNA and 4 other plasmids that encode individually the VSV N, P, G and L proteins, all of which are under control of T7 promoters.
From the laboratory of Michael A. Whitt, Ph.D., University of Tennessee.
Part of The Investigator's Annexe program.
The plasmid pBS-G-ΦT encodes the vesicular stomatitis virus (VSV) glycoprotein (G) from the Indiana serotype expressed from the bacteriophage T7 promoter in pBS, which has been further modified to contain a T7 terminator sequence cloned between the SacII and SacI restriction sites in pBS-SK+ [1]. The plasmid is used as part of the VSV reverse genetics system to recover replication-restricted ΔG-VSV from plasmids [2]. The recovery of ΔG-VSV involves the transfection of cells that have been infected with recombinant vaccinia expressing bacteriophage T7 RNA polymerase (vvT7) with 5 different plasmids; one which expresses the ΔG-VSV antigenomic-sense (or positive-sense) RNA and 4 other plasmids that encode individually the VSV N, P, G and L proteins, all of which are under control of T7 promoters.
From the laboratory of Michael A. Whitt, Ph.D., University of Tennessee.
Part of The Investigator's Annexe program.
| Product Type: | Plasmid |
| Gene/insert name: | VSV-Indiana glycoprotein (G) |
| Accession ID: | P03522 |
| Antibiotic Resistance: | Ampicillin or Kanamycin (please see vial label and packing slip) |
| Fusion Tag(s): | none |
| Concentration: | 100uL (100ng/uL) |
| Grow in E. coli at 37 C: | Yes |
| Cloning Site 5': | ApaI |
| Cloning Site 3': | BamHI |
| Insert Size: | 1,700 bp |
| Vector Backbone and Size: | pBS-SK-ΦT, 3105 bp |
| High or low copy: | High |
| Comments: | For suggested protocol, see: Whitt, MA, J. Virol. Methods, 2010. 169(2): p. 365-74. |
| Shipped: | Ambient temperature |
- Stillman, E.A., J.K. Rose, and M.A. Whitt, Replication and amplification of novel vesicular stomatitis virus minigenomes encoding viral structural proteins. J. Virol., 1995. 69: p. 2946-2953.
- Whitt, M.A., Generation of VSV pseudotypes using recombinant DeltaG-VSV for studies on virus entry, identification of entry inhibitors, and immune responses to vaccines. J. Virol. Methods, 2010. 169(2): p. 365-74.
- Kane M, Deiss F, Chervonsky A, Golovkina TV. A Single Locus Controls Interferon Gamma-Independent Antiretroviral Neutralizing Antibody Responses. J Virol. 2018 Jul 31;92(16). pii: e00725-18. doi: 10.1128/JVI.00725-18. Print 2018 Aug 15. View Article
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