Anti-Cytoplasmic Dynein [74.1] Antibody
![Anti-Cytoplasmic Dynein [74.1] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1046_2001202001263210450.jpg "Anti-Cytoplasmic Dynein [74.1] Antibody")
This mouse IgG2b antibody was generated against bovine brain cytoplasmic dynein and recognizes an epitope in the N-terminal 60 amino acids of the intermediate chain (IC) subunit of cow, mouse, rat, dog, sheep, fish, xenopus, and drosophila cytoplasmic dynein homologs.
Highlights:
- Reacts with an epitope in the N-terminal 60 amino acids of the intermediate chain (IC) subunit of cow, mouse, rat, dog, sheep, fish, xenopus, and drosophila cytoplasmic dynein homologs
- Suitable for Western Blot, Immunoprecipitation and Immunofluorescence applications
Eukaryotic cells rely on actin and microtubule-based protein "motors" to generate intracellular movements. These motor proteins contain specialized domains that hydrolyse ATP to produce force and movement along a cytoskeletal polymer (microtubules in the case of the kinesin family and dyneins). In all cell types, dynein is a large protein complex composed of six distinct subunits and is responsible for most intracellular movement toward the minus ends of microtubules. Cytoplasmic dynein has many important roles in transporting membrane bound organelles, including endosomes, lysosomes, and mitochondria. It is also involved in the positioning of the centrosome, the nucleus, and the Golgi. It is comprised of 2 heavy chain polypeptides (DYNC1H1, DYNC2H1), two intermediate chains (DYNC1I1, DYNC1I2), light-intermediate chains (DYNC1LI1, DYNC1LI2) and light chains (DYNLT1, DYNLT3, DYNLRB1, DYNLRB2, DYNLL1, DYNLLl2). Cytoplasmic dynein is also responsible for virus transport to the nucleus, retrograde axonal transport, and microtubule and neurofilament transport.
From a laboratory at University of Virginia.
This mouse IgG2b antibody was generated against bovine brain cytoplasmic dynein and recognizes an epitope in the N-terminal 60 amino acids of the intermediate chain (IC) subunit of cow, mouse, rat, dog, sheep, fish, xenopus, and drosophila cytoplasmic dynein homologs.
Highlights:
- Reacts with an epitope in the N-terminal 60 amino acids of the intermediate chain (IC) subunit of cow, mouse, rat, dog, sheep, fish, xenopus, and drosophila cytoplasmic dynein homologs
- Suitable for Western Blot, Immunoprecipitation and Immunofluorescence applications
Eukaryotic cells rely on actin and microtubule-based protein "motors" to generate intracellular movements. These motor proteins contain specialized domains that hydrolyse ATP to produce force and movement along a cytoskeletal polymer (microtubules in the case of the kinesin family and dyneins). In all cell types, dynein is a large protein complex composed of six distinct subunits and is responsible for most intracellular movement toward the minus ends of microtubules. Cytoplasmic dynein has many important roles in transporting membrane bound organelles, including endosomes, lysosomes, and mitochondria. It is also involved in the positioning of the centrosome, the nucleus, and the Golgi. It is comprised of 2 heavy chain polypeptides (DYNC1H1, DYNC2H1), two intermediate chains (DYNC1I1, DYNC1I2), light-intermediate chains (DYNC1LI1, DYNC1LI2) and light chains (DYNLT1, DYNLT3, DYNLRB1, DYNLRB2, DYNLL1, DYNLLl2). Cytoplasmic dynein is also responsible for virus transport to the nucleus, retrograde axonal transport, and microtubule and neurofilament transport.
From a laboratory at University of Virginia.
| Product Type: | Antibody |
| Name: | Anti-Cytoplasmic Dynein [74.1] Antibody |
| Antigen: | Cytoplasmic Dynein |
| Accession ID: | Q14204 |
| Isotype: | IgG2b |
| Clonality: | Monoclonal |
| Clone Name: | 74.1 |
| Reactivity: | Cow, mouse, rat, dog, sheep, fish, xenopus, drosophila |
| Immunogen: | Generated against bovine brain cytoplasmic dynein |
| Species Immunized: | Mouse |
| Epitope: | N-terminal 60 amino acids of the intermediate chain (IC) subunit of cow, mouse, rat, dog, sheep, fish, xenopus, and drosophila homologs |
| Purification Method: | Protein A or A/G affinity purified |
| Buffer: | PBS, 0.05% Sodium Azide |
| Tested Applications: | WB, IP, IF |
| Storage: | -20C |
| Shipped: | Cold packs |
- Dillman JF 3rd, Pfister KK. Differential phosphorylation in vivo of cytoplasmic dynein associated with anterogradely moving organelles. J Cell Biol. 1994 Dec;127(6 Pt 1):1671-81. doi: 10.1083/jcb.127.6.1671. PMID: 7528220; PMCID: PMC2120311.
If you publish research with this product, please let us know so we can cite your paper.
