Scomber scombrus Myoblast Cell Line (MACK1)
MACK1 (myoblast cell) is a continuous, adherent cell line derived from mature muscle of Atlantic mackerel (Scomber scombrus).
Highlights:
- Cell line is useful for cellular agriculture
- Cells progressed through a likely spontaneous immortalization crisis event, after which the cell doubling time decreased to ~30-35 hours
- PCR and Sanger Sequencing on the cells' genomic DNA confirmed cells' identity as Scomber scombrus
- Highly pure satellite cell population confirmed through Pax7 staining and efficient muscle differentiation
MACK1 is the first commercially available fish muscle cell line and can prove a valuable tool for studying marine cell culture. As Atlantic mackerel is a commonly consumed fish, the food-relevant cell line is especially valuable in cultivated seafood applications.
From the laboratory of David Kaplan, PhD, Tufts University Center for Cellular Agriculture (TUCCA).
MACK1 (myoblast cell) is a continuous, adherent cell line derived from mature muscle of Atlantic mackerel (Scomber scombrus).
Highlights:
- Cell line is useful for cellular agriculture
- Cells progressed through a likely spontaneous immortalization crisis event, after which the cell doubling time decreased to ~30-35 hours
- PCR and Sanger Sequencing on the cells' genomic DNA confirmed cells' identity as Scomber scombrus
- Highly pure satellite cell population confirmed through Pax7 staining and efficient muscle differentiation
MACK1 is the first commercially available fish muscle cell line and can prove a valuable tool for studying marine cell culture. As Atlantic mackerel is a commonly consumed fish, the food-relevant cell line is especially valuable in cultivated seafood applications.
From the laboratory of David Kaplan, PhD, Tufts University Center for Cellular Agriculture (TUCCA).
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Specifications
| Product Type: | Cell Line |
| Name: | MACK1 |
| Cell Type: | Muscle cell line |
| Organism: | Atlantic mackerel |
| Source: | White muscle |
| Biosafety Level: | BSL-2 |
| Growth Conditions: |
Growth media formulation: Leibovitz's L-15 Medium with 20% fetal bovine serum (FBS), 20 mM HEPES pH7.4, 1 ng/mL FGF-2, 1% antibiotic-antimycotic, and 10 µg/mL gentamicin. Recommend to add ECM mimetic, such as laminin (typically at 0.25 μg/cm2), (pre-coating or in media) to improve cell adhesion. Culture the cells at 27°C without CO2 in vented cell culture flasks. For reference, the providing lab uses the following type of incubator: Thermo Scientific Cat. No. 50125590 With the first thaw, plate all 1 million cells in the vial into a 25 cm2 tissue culture flask. Allow cells 1-2 days to recover before changing the medium. The high seeding density is necessary due to poor cell viability after freezing/thawing. |
| Subculturing: |
Split with 0.05% or 0.25% trypsin-EDTA when cells reach 70-80% confluence. Recommended seeding density: 3,000-6,000 cells/cm2. It is further recommended to add ECM mimetic, such as laminin (typically at 0.25 μg/cm2), (pre-coating or in media) to improve cell adhesion. |
| Cryopreservation: | Growth media with 10% DMSO |
| Comments: |
Doubling time: ~30-35 hours.; For thawing cells, seed the cells in a T25 tissue culture flask. The high seeding density is necessary due to poor cell viability after freezing/thawing. NOTE: The cells are difficult to visualize on brightfield microscopy. Phase contrast is recommended. |
| Storage: | LN2 |
| Shipped: | Dry ice |
Provider
From the laboratory of David Kaplan, PhD, Tufts University Center for Cellular Agriculture (TUCCA).
References
- Saad MK, Yuen JSK Jr, Joyce CM, et al. Continuous fish muscle cell line with capacity for myogenic and adipogenic-like phenotypes. Sci Rep. 2023;13(1):5098. Published 2023 Mar 29. doi:10.1038/s41598-023-31822-2
- Michael K Saad, John SK Yuen Jr, Connor M Joyce, Xinxin Li, Taehwan Lim, Talia L Wolfson, Justin Wu, Jason Laird, Sanjana Vissapragada, Olivia P Calkins, Adham Ali, David L Kaplan. Continuous Fish Muscle Cell Line with Capacity for Myogenic and Adipogenic-like Phenotypes. bioRxiv 2022.08.22.504874; doi: https://doi.org/10.1101/2022.08.22.504874
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