Anti-H3K9me3 Antibody
This H3K9me3 rabbit polyclonal antibody was raised against a modified branched peptide and specifically recognizes histone H3 tri-methylated on lysine 9 (H3K9me3).
Highlights:
- Detects a single protein of approximately 14 kDa by western blot using acid extracted histones
- Suitable for chromatin immunoprecipitation (ChIP) assays
H3K9me3 indicates the addition of a trimethyl group to the 9th lysine residue on the histone H3 protein, and is often associated with heterochromatin. This post-translation modification is located on the amino (N) terminal charged tails of the histone and plays an important role in many biological processes, such as in embryonic stem cells at the onset of organogenesis.
This H3K9me3 rabbit polyclonal antibody was raised against a modified branched peptide and specifically recognizes histone H3 tri-methylated on lysine 9 (H3K9me3).
Highlights:
- Detects a single protein of approximately 14 kDa by western blot using acid extracted histones
- Suitable for chromatin immunoprecipitation (ChIP) assays
H3K9me3 indicates the addition of a trimethyl group to the 9th lysine residue on the histone H3 protein, and is often associated with heterochromatin. This post-translation modification is located on the amino (N) terminal charged tails of the histone and plays an important role in many biological processes, such as in embryonic stem cells at the onset of organogenesis.
Specifications
| Product Type: | Antibody |
| Antigen: | H3K9me3 |
| Clonality: | Polyclonal |
| Clone Name: | H3K9me3 |
| Immunogen: | Modified branched peptide |
| Species Immunized: | Rabbit |
| Buffer: | Whole serum |
| Tested Applications: | ChIP assay (ideal for ChIP-seq or ChIP-qPCR) |
| Comments: | ChIP Recommendations: Use 2.5-5.0 ul of crude sera with 40-60 ul of Protein A beads per 100 ug of sheared chromatin lysate. We recommend titrating the antibody, beads, and lysate to determine ideal concentrations. We also recommend pre-binding the antibody to beads. |
| Storage: | -80C |
| Shipped: | Cold Packs (Domestic, Overnight); Dry Ice (International) |
Provider
From the laboratory at Apheon Bioscience.
Comments
The antibody detects a single protein of approximately 14 kDa by western blot using acid extracted histones. The antibody has been tested in chromatin immunoprecipitation (ChIP) experiments against a strain lacking all H3K9 methyltransferase activity and determined to be specific.
References
If you publish research with this product, please let us know so we can cite your paper.
