This goat IgG polyclonal antibody was generated against peptide sequence sequence C-GVDEFSTLVAES, from the C Terminus of the Parvalbumin (PVALB) protein sequence according to NP_002845.1, and recognizes human and rat PVALB .
Highlights:
Parvalbumin (PVALB) is a calcium-binding protein that is part of the EF-hand superfamily. It is structurally and functionally related to calmodulin and troponin C and found in fast-contracting muscles. Parvalbumin plays an important role in signal transduction in tissues such as muscles and neurons. This protein may play a role in diseases such as schizophrenia as well as food allergies.
Product Type: | Antibody |
Name: | Goat Anti-Parvalbumin Antibody |
Accession ID: | NP_002845.1 |
Antigen: | Parvalbumin |
Isotype: | IgG |
Clonality: | Polyclonal |
Reactivity: | Human and Rat |
Specificity: | Parvalbumin |
Immunogen: | CGVDEFSTLVAES |
Species Immunized: | Goat |
Epitope: | C Terminus |
Purification Method: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Buffer: | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
Tested Applications: | Pep-ELISA, WB, FC |
Storage: | Aliquot and store at -20C. Minimize freezing and thawing. |
Shipped: | Cold Packs |
Western Blot
(0.01µg/ml) staining of Human Cerebellum (A) lysate + peptide (B) and (0.3ug/ml) Rat Brain (C) lysate + peptide (D) (35µg protein in RIPA buffer). Detected by chemiluminescence.
Flow Cytometry
Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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