Goat Anti-Asporin / ASPN Antibody
This goat IgG polyclonal antibody was generated against peptide sequence C-IHENKVKKIQKD from the internal region of asporin (ASPN) and recognizes human, mouse, and rat ASPN.
Highlights:
- Reacts with human, mouse, and rat ASPN
- Suitable for Peptide ELISA, Western Blot, and Immunofluorescence applications
- Polymorphisms are associated with osteoarthritis and intervertebral disc disease
Asporin (ASPN) is an extracellular matrix protein that is a member of the small leucine-rich proteoglycan family of proteins. ASPN is present in bone, cartilage, and the periodontal ligament. ASPN has a unique aspartic acid repeat at the N terminus of the mature protein. Polymorphisms in the aspartic acid repeat region of the gene are associated with osteoarthritis and intervertebral disc disease.
This goat IgG polyclonal antibody was generated against peptide sequence C-IHENKVKKIQKD from the internal region of asporin (ASPN) and recognizes human, mouse, and rat ASPN.
Highlights:
- Reacts with human, mouse, and rat ASPN
- Suitable for Peptide ELISA, Western Blot, and Immunofluorescence applications
- Polymorphisms are associated with osteoarthritis and intervertebral disc disease
Asporin (ASPN) is an extracellular matrix protein that is a member of the small leucine-rich proteoglycan family of proteins. ASPN is present in bone, cartilage, and the periodontal ligament. ASPN has a unique aspartic acid repeat at the N terminus of the mature protein. Polymorphisms in the aspartic acid repeat region of the gene are associated with osteoarthritis and intervertebral disc disease.
| Product Type: | Antibody |
| Name: | Goat Anti-Asporin / ASPN Antibody |
| Alternative Name(s): | Periodontal ligament-associated protein 1, PLAP-1, SLRR1C |
| Accession ID: | NP_060150.4 |
| Antigen: | Asporin / ASPN |
| Isotype: | IgG |
| Clonality: | Polyclonal |
| Reactivity: | Human, Mouse, Rat |
| Specificity: | Asporin / ASPN |
| Immunogen: | CIHENKVKKIQKDT |
| Species Immunized: | Goat |
| Epitope: | Internal Region |
| Purification Method: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide |
| Buffer: | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Tested Applications: | Pep-ELISA, WB, IF |
| Storage: | Aliquot and store at -20C. Minimize freezing and thawing. |
| Shipped: | Cold Packs |
Western Blot
(0.1µg/ml) staining of Human Tonsil (A), (0.3ug/ml) Human Uterus (B), Mouse Skeletal Muscle (C) (1ug/ml) Rat Skeletal Muscle (D) and negative control Human Cerebellum (E) lysate (35µg protein in RIPA buffer).Detected by chemiluminescence.
Immunofluorescence
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
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