Goat Anti-SOX2 Antibody
This goat IgG polyclonal antibody was generated against peptide sequence YNMMETELKPPGPQQC from the N Terminus of SRY-box transcription factor 2 (SOX2) and recognizes human SOX2.
Highlights:
- Reacts with human SOX2
- Suitable for Peptide ELISA, Western Blot, Immunohistochemistry, and Flow Cytometry applications
- Mutations are associated with optic nerve hypoplasia and syndromic microphthalmia
SRY-box transcription factor 2 (SOX2) is a member of the Sox family of transcription factors that are regulators of cell fate during development. SOX2 is critical for early embryonic development and for embryonic stem cell pluripotency. Mutations in this gene have been associated with optic nerve hypoplasia and syndromic microphthalmia.
This goat IgG polyclonal antibody was generated against peptide sequence YNMMETELKPPGPQQC from the N Terminus of SRY-box transcription factor 2 (SOX2) and recognizes human SOX2.
Highlights:
- Reacts with human SOX2
- Suitable for Peptide ELISA, Western Blot, Immunohistochemistry, and Flow Cytometry applications
- Mutations are associated with optic nerve hypoplasia and syndromic microphthalmia
SRY-box transcription factor 2 (SOX2) is a member of the Sox family of transcription factors that are regulators of cell fate during development. SOX2 is critical for early embryonic development and for embryonic stem cell pluripotency. Mutations in this gene have been associated with optic nerve hypoplasia and syndromic microphthalmia.
| Product Type: | Antibody |
| Name: | Goat Anti-SOX2 Antibody |
| Alternative Name(s): | ANOP3, MCOPS3 |
| Accession ID: | NP_003097.1 |
| Antigen: | SOX2 |
| Isotype: | IgG |
| Clonality: | Polyclonal |
| Reactivity: | Human |
| Specificity: | SOX2 |
| Immunogen: | YNMMETELKPPGPQQC |
| Species Immunized: | Goat |
| Epitope: | N Terminus |
| Purification Method: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide |
| Buffer: | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Tested Applications: | Pep-ELISA, WB, IHC, FC |
| Storage: | Aliquot and store at -20C. Minimize freezing and thawing. |
| Shipped: | Cold Packs |
Western Blot, Flow Cytometry, and Immunohistochemistry
I. (0.3µg/ml) staining of MCF7 (A) HEK293 (B) and MCF7 nuclear (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence. II. Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. III. (8µg/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. IV. Negative control showing staining of paraffin embedded Human Tonsil with no primary antibody.
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