Goat Anti-Doublecortin / DCX (aa232-242) Antibody
This goat IgG polyclonal antibody was generated against peptide sequence CKTSANMKAPQS from the internal region of neuronal migration protein doublecortin (DCX) and recognizes human, mouse, and rat DCX.
Highlights:
- Reacts with human, mouse, and rat DCX
- Suitable for Peptide ELISA, Western Blot, Immunohistochemistry, and Immunofluorescence applications
- Mutations in the DCX gene result in lissencephaly or subcortical band heterotopia
Neuronal migration protein doublecortin (DCX) is a microtubule-associated protein that is involved in neuronal migration in embryonic and postnatal brain development. DCX regulates the stability and organization of microtubules by influencing microtuble rigidity, curvature, and the 13-protofilament geometry. Mutations in the DCX gene can lead to epilepsy, cognitive disability, subcortical band heterotopia, and lissencephaly.
This goat IgG polyclonal antibody was generated against peptide sequence CKTSANMKAPQS from the internal region of neuronal migration protein doublecortin (DCX) and recognizes human, mouse, and rat DCX.
Highlights:
- Reacts with human, mouse, and rat DCX
- Suitable for Peptide ELISA, Western Blot, Immunohistochemistry, and Immunofluorescence applications
- Mutations in the DCX gene result in lissencephaly or subcortical band heterotopia
Neuronal migration protein doublecortin (DCX) is a microtubule-associated protein that is involved in neuronal migration in embryonic and postnatal brain development. DCX regulates the stability and organization of microtubules by influencing microtuble rigidity, curvature, and the 13-protofilament geometry. Mutations in the DCX gene can lead to epilepsy, cognitive disability, subcortical band heterotopia, and lissencephaly.
| Product Type: | Antibody |
| Name: | Goat Anti-Doublecortin / DCX (aa232-242) Antibody |
| Alternative Name(s): | Doublin, Lissencephalin-X (Lis-X) |
| Accession ID: | NP_000546.2; NP_835365.1; NP_835364.1; NP_001182482.1 |
| Antigen: | Doublecortin / DCX (aa232-242) |
| Isotype: | IgG |
| Clonality: | Polyclonal |
| Reactivity: | Human, Mouse, Rat |
| Specificity: | Doublecortin / DCX (aa232-242) |
| Immunogen: | CKTSANMKAPQS |
| Species Immunized: | Goat |
| Epitope: | Internal Region |
| Purification Method: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide |
| Buffer: | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Tested Applications: | Pep-ELISA, WB, IHC, IF |
| Storage: | Aliquot and store at -20C. Minimize freezing and thawing. |
| Shipped: | Cold Packs |
Western Blot, Immunofluorescence, and Immunohistochemistry
I. (0.01µg/ml) staining of Mouse fetal Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence. II. Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). III. Immunofluorescence analysis of paraformaldehyde fixed KNRK cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IV. (2µg/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. V. Negative control showing staining of paraffin embedded Human Cerebellum with no primary antibody.
- Heysieattalab, S., Sadeghi, Dynamic structural neuroplasticity during and after epileptogenesis in a pilocarpine rat model of epilepsy, Acta Epileptologica 3, 3 (2021). https://doi.org/10.1186/s42494-020-00037-7 ,0
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