Immortalized Bovine Satellite Cell Line (iBSC1)
iBSC1 is an immortalized bovine satellite cell line, isolated from the semitendinosus muscle of a female Simmental calf (bos taurus). The cells were generated through Sleeping Beauty transposon-mediated insertion of bovine TERT and CDK4 under constitutive promotion by CMV and RPBSA, respectively.
Highlights:
- Contains a puromycin resistance gene
- Overexpresses bovine TERT and CDK4
- Useful for cellular agriculture and cultured meat applications
- Offers robust proliferation while maintaining its myogenic phenotype
From the laboratory of David Kaplan, PhD, Tufts University Center for Cellular Agriculture (TUCCA).
iBSC1 is an immortalized bovine satellite cell line, isolated from the semitendinosus muscle of a female Simmental calf (bos taurus). The cells were generated through Sleeping Beauty transposon-mediated insertion of bovine TERT and CDK4 under constitutive promotion by CMV and RPBSA, respectively.
Highlights:
- Contains a puromycin resistance gene
- Overexpresses bovine TERT and CDK4
- Useful for cellular agriculture and cultured meat applications
- Offers robust proliferation while maintaining its myogenic phenotype
From the laboratory of David Kaplan, PhD, Tufts University Center for Cellular Agriculture (TUCCA).
Specifications
| Product Type: | Cell Line |
| Name: | iBSC1 |
| Cell Type: | Muscle satellite cells |
| Morphology: | Myogenic |
| Organism: | Bos taurus |
| Source: | Semitendinosus muscle |
| Biosafety Level: | BSL-1 |
| Subculturing: | Doubling time: ~15 hours. Subculture at ~70% confluency. Plate ~2000/cm^2. |
| Growth Conditions: |
DMEM high glucose, with glutamax (such as such as ThermoFisher #10566024)+ 20% FBS + 1 ng/mL FGF-2 + 2.5 ug/mL puromycin The providing lab strongly urges being very quick with thawing/resuspending of the cells from the cryovials, and spinning-down before plating (rather than plating in the diluted freezing medium). |
| Cryopreservation: | Growth media with 10% DMSO |
| Comments: |
The providing lab recommends culturing the cells on Laminin-511 coated plates (0.5 ug/cm^2). It is further recommended that users grow the cells for 2-3 passages, by trypsinizing and replating, after thawing the initial vial and before freezing stock vials for further use. Contains a puromycin resistance gene. To differentiate, culture cells to confluency and change to differentiation medium (e.g., DMEM + 2% FBS). |
| Storage: | LN2 |
| Shipped: | Dry ice |
Provider
From the laboratory of David Kaplan, PhD, Tufts University Center for Cellular Agriculture (TUCCA).
References
- Stout AJ, Arnett MJ, Chai K, et al. Immortalized Bovine Satellite Cells for Cultured Meat Applications. ACS Synth Biol. 2023;12(5):1567-1573. doi:10.1021/acssynbio.3c00216
If you publish research with this product, please let us know so we can cite your paper.
