NIH3T3/3790#30 Cell Line
NIH3T3/3790#30 is a mitochondrial DNA lacking cell line derived from the NIH3T3 cells through transient transfection with a plasmid encoding EGFP and UNG1 Y147A mutant.
Highlights:
Lacks mitochondrial DNA (rho-θ, ρ)
Can be used as a recipient of mtDNA
May be useful for studies related to the oxidative phosphorylation system or the effects of different mtDNA mutations (as recipient mtDNA)
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.
NIH3T3/3790#30 is a mitochondrial DNA lacking cell line derived from the NIH3T3 cells through transient transfection with a plasmid encoding EGFP and UNG1 Y147A mutant.
- Highlights:
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.
Specifications
| Product Type: | Cell Line |
| Name: | NIH3T3/3790#30 Cell Line |
| Cell Type: | Mouse embryonic fibroblasts |
| Morphology: | Fibroblast |
| Organism: |
|
| Source: | Mouse embryo |
| Biosafety Level: | BSL-1 |
| Growth Conditions: | DMEM with 4.5 g/l glucose, 10% FBS (such as Biowest, Cat. No. S1620), 1 mM sodium pyruvate, 50 ug/ml uridine |
| Subculturing: | Split 1:4 to 1:8, 1-2 times/week |
| Cryopreservation: | 45% media, 45% newborn calf serum (such as Fisher Cat. No. NC1928575), 10% DMSO |
| Comments: | Slow growth, may survive poorly after freezing |
| Storage: | LN2 |
| Shipped: | Dry Ice |
Provider
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.
Comments
NIH3T3/3790#30: Transiently transfected NIH3T3 cells with a plasmid encoding EGFP and UNG1 Y147A mutant were sorted for EGFP fluorescence. Cloning was described in PMID: 36809687.
References
- Khozhukhar N, Spadafora D, Rodriguez YAR, Fayzulin R, Alexeyev M. Generation of Mammalian Cells Devoid of Mitochondrial DNA (?0 cells). Curr Protoc. 2023 Feb;3(2):e679. doi: 10.1002/cpz1.679. PMID: 36809687; PMCID: PMC10151036.
- Spadafora D, Kozhukhar N, Chouljenko VN, Kousoulas KG, Alexeyev MF. Methods for Efficient Elimination of Mitochondrial DNA from Cultured Cells. PLoS One. 2016 May 2;11(5):e0154684. doi: 10.1371/journal.pone.0154684. PMID: 27136098; PMCID: PMC4852919.
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